Abstract
We have determined the cistrome and transcriptome for the nuclear receptor liver receptor homolog-1 (LRH-1) in exocrine pancreas. Chromatin immunoprecipitation (ChIP)-seq and RNA-seq analyses reveal that LRH-1 directly induces expression of genes encoding digestive enzymes and secretory and mitochondrial proteins. LRH-1 cooperates with the pancreas transcription factor 1-L complex (PTF1-L) in regulating exocrine pancreas-specific gene expression. Elimination of LRH-1 in adult mice reduced the concentration of several lipases and proteases in pancreatic fluid and impaired pancreatic fluid secretion in response to cholecystokinin. Thus, LRH-1 is a key regulator of the exocrine pancreas-specific transcrip-tional network required for the production and secretion of pancreatic fluid.
Original language | English (US) |
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Pages (from-to) | 1674-1679 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 25 |
Issue number | 16 |
DOIs | |
State | Published - Aug 15 2011 |
Keywords
- ChIP-seq
- Digestive enzymes
- Exocrine pancreas
- LRH-1
- PTF1-L
- RNA-seq
ASJC Scopus subject areas
- Genetics
- Developmental Biology