LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines

Chun Xiang Liu, Simone Musco, Natalia M. Lisitsina, Eva Forgacs, John D. Minna, Nikolai A. Lisitsyn

Research output: Contribution to journalArticle

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Abstract

A variety of studies suggest that allelic losses at chromosome 2q are associated with aggressive behavior of various forms of human neoplasia. Using a probe to detect homozygous deletions on chromosome 2q21.2 in kidney and bladder cancer cell lines, we identified a new candidate tumor suppressor gene, lipoprotein receptor-related protein-deleted in tumors (LRP-DIT). The predicted LRP-DIT product of 4599 amino acids has extensive homology to a gigantic receptor, LRP1, which mediates endocytosis of multiple proteins from the cell surface. Homozygous deletions in LRP-DIT were detected in 17% (4 of 23) of non-small cell lung cancer (NSCLC) cell lines. The expression of only abnormal transcripts missing portions of the LRP-DIT sequence was demonstrated in an additional 30 % (11 of 36) of NSCLC lines. Finally, a missense mutation at codon 3157 was detected in one of four NSCLC lines tested for the large open reading frame. In contrast, no LRP-DIT alterations were identified in a major fraction of SCLC cell lines, indicating that this gene is preferentially inactivated in one histological type of lung cancer. Our data suggest that inactivation of LRP-DIT occurs in at least 40% of NSCLC lines and thus may play an important role in tumorigenesis of NSCLCs.

Original languageEnglish (US)
Pages (from-to)1961-1967
Number of pages7
JournalCancer Research
Volume60
Issue number7
StatePublished - Apr 1 2000

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Lipoprotein Receptors
Non-Small Cell Lung Carcinoma
Cell Line
Genes
Neoplasms
Proteins
Chromosome Deletion
Kidney Neoplasms
Loss of Heterozygosity
Missense Mutation
Endocytosis
Tumor Suppressor Genes
Urinary Bladder Neoplasms
Codon
Open Reading Frames
Lung Neoplasms
Membrane Proteins
Carcinogenesis
Chromosomes
Amino Acids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Liu, C. X., Musco, S., Lisitsina, N. M., Forgacs, E., Minna, J. D., & Lisitsyn, N. A. (2000). LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Research, 60(7), 1961-1967.

LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. / Liu, Chun Xiang; Musco, Simone; Lisitsina, Natalia M.; Forgacs, Eva; Minna, John D.; Lisitsyn, Nikolai A.

In: Cancer Research, Vol. 60, No. 7, 01.04.2000, p. 1961-1967.

Research output: Contribution to journalArticle

Liu, CX, Musco, S, Lisitsina, NM, Forgacs, E, Minna, JD & Lisitsyn, NA 2000, 'LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines', Cancer Research, vol. 60, no. 7, pp. 1961-1967.
Liu, Chun Xiang ; Musco, Simone ; Lisitsina, Natalia M. ; Forgacs, Eva ; Minna, John D. ; Lisitsyn, Nikolai A. / LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. In: Cancer Research. 2000 ; Vol. 60, No. 7. pp. 1961-1967.
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