LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines

Chun Xiang Liu, Simone Musco, Natalia M. Lisitsina, Eva Forgacs, John D. Minna, Nikolai A. Lisitsyn

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

A variety of studies suggest that allelic losses at chromosome 2q are associated with aggressive behavior of various forms of human neoplasia. Using a probe to detect homozygous deletions on chromosome 2q21.2 in kidney and bladder cancer cell lines, we identified a new candidate tumor suppressor gene, lipoprotein receptor-related protein-deleted in tumors (LRP-DIT). The predicted LRP-DIT product of 4599 amino acids has extensive homology to a gigantic receptor, LRP1, which mediates endocytosis of multiple proteins from the cell surface. Homozygous deletions in LRP-DIT were detected in 17% (4 of 23) of non-small cell lung cancer (NSCLC) cell lines. The expression of only abnormal transcripts missing portions of the LRP-DIT sequence was demonstrated in an additional 30 % (11 of 36) of NSCLC lines. Finally, a missense mutation at codon 3157 was detected in one of four NSCLC lines tested for the large open reading frame. In contrast, no LRP-DIT alterations were identified in a major fraction of SCLC cell lines, indicating that this gene is preferentially inactivated in one histological type of lung cancer. Our data suggest that inactivation of LRP-DIT occurs in at least 40% of NSCLC lines and thus may play an important role in tumorigenesis of NSCLCs.

Original languageEnglish (US)
Pages (from-to)1961-1967
Number of pages7
JournalCancer Research
Volume60
Issue number7
StatePublished - Apr 1 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Liu, C. X., Musco, S., Lisitsina, N. M., Forgacs, E., Minna, J. D., & Lisitsyn, N. A. (2000). LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Research, 60(7), 1961-1967.