Lymphotoxin-β receptor-dependent genes in lymph node and follicular dendritic cell transcriptomes

Christoph Huber, Caroline Thielen, Harald Seeger, Petra Schwarz, Fabio Montrasio, Mark R. Wilson, Ernst Heinen, Yang Xin Fu, Gino Miele, Adriano Aguzzi

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Affinity maturation and Ab class switches occur in lymphoid germinal centers (GCs), in which differentiation and maintenance depend on lymphotoxin (LT) signaling and include differentiation of follicular dendritic cells (FDCs). The events leading to FDC and GC maturation are poorly defined. Using several approaches of functional genomics, we enumerated transcripts affected in mice by suppressing LT β receptor (LTβR) signaling and/or overrepresented in FDC-enriched GC isolates. Protein expression analysis of 3 of 12 genes both enriched in FDCs and down-regulated by LTβR signaling suppression validated them as FDC markers. Functional analysis of one of these three, clusterin, suggests a role as an FDC-derived trophic factor for GC B cells. Hence, the set of genes presented in this study includes markers emanating from LTβR signaling and transcripts relevant to GC and FDC function.

Original languageEnglish (US)
Pages (from-to)5526-5536
Number of pages11
JournalJournal of Immunology
Volume174
Issue number9
StatePublished - May 1 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Huber, C., Thielen, C., Seeger, H., Schwarz, P., Montrasio, F., Wilson, M. R., Heinen, E., Fu, Y. X., Miele, G., & Aguzzi, A. (2005). Lymphotoxin-β receptor-dependent genes in lymph node and follicular dendritic cell transcriptomes. Journal of Immunology, 174(9), 5526-5536.