Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis

Sabina Sangaletti, Emma Di Carlo, Silvia Gariboldi, Silvia Miotti, Barbara Cappetti, Mariella Parenza, Cristiano Rumio, Rolf A. Brekken, Claudia Chiodoni, Mario P. Colombo

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Other than genetic imprinting and epithelial to mesenchymal transition, cancer cells need interaction with the nearby stroma toward metastasis. Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein known to regulate extracellular matrix (ECM) deposition and cell-ECM interaction. Gene expression profiles associate SPARC to malignant progression. Using reciprocal bone marrow chimeras between SPARC knockout and wild-type mice, we show that SPARC produced by inflammatory cells is necessary for spontaneous, but not experimental, i.v. metastasis. Macrophage-derived SPARC induces cancer cell migration and enhances their migration to other ECM proteins at least through αvβ5 integrin. Indeed, RNA interference knockdown of β5 integrin expression reduces cell migration in vitro and metastasis in vivo. Together these results show that macrophage-derived SPARC takes part in metastasis, acting at the step of integrin-mediated migration of invasive cells.

Original languageEnglish (US)
Pages (from-to)9050-9059
Number of pages10
JournalCancer Research
Volume68
Issue number21
DOIs
StatePublished - Nov 1 2008

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Extracellular Matrix
Cysteine
Macrophages
Neoplasm Metastasis
Neoplasms
Integrins
Proteins
Cell Movement
Genomic Imprinting
Epithelial-Mesenchymal Transition
Extracellular Matrix Proteins
RNA Interference
Transcriptome
Knockout Mice
Cell Communication
Bone Marrow

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sangaletti, S., Di Carlo, E., Gariboldi, S., Miotti, S., Cappetti, B., Parenza, M., ... Colombo, M. P. (2008). Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Research, 68(21), 9050-9059. https://doi.org/10.1158/0008-5472.CAN-08-1327

Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. / Sangaletti, Sabina; Di Carlo, Emma; Gariboldi, Silvia; Miotti, Silvia; Cappetti, Barbara; Parenza, Mariella; Rumio, Cristiano; Brekken, Rolf A.; Chiodoni, Claudia; Colombo, Mario P.

In: Cancer Research, Vol. 68, No. 21, 01.11.2008, p. 9050-9059.

Research output: Contribution to journalArticle

Sangaletti, S, Di Carlo, E, Gariboldi, S, Miotti, S, Cappetti, B, Parenza, M, Rumio, C, Brekken, RA, Chiodoni, C & Colombo, MP 2008, 'Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis', Cancer Research, vol. 68, no. 21, pp. 9050-9059. https://doi.org/10.1158/0008-5472.CAN-08-1327
Sangaletti S, Di Carlo E, Gariboldi S, Miotti S, Cappetti B, Parenza M et al. Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Research. 2008 Nov 1;68(21):9050-9059. https://doi.org/10.1158/0008-5472.CAN-08-1327
Sangaletti, Sabina ; Di Carlo, Emma ; Gariboldi, Silvia ; Miotti, Silvia ; Cappetti, Barbara ; Parenza, Mariella ; Rumio, Cristiano ; Brekken, Rolf A. ; Chiodoni, Claudia ; Colombo, Mario P. / Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. In: Cancer Research. 2008 ; Vol. 68, No. 21. pp. 9050-9059.
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