Mannose-6-phosphate receptors (MPR 300 and 46) from the highly evolved invertebrate Asterias rubens (Echinodermate): Biochemical and functional characterization of MPR 46 protein

Sivaramakrishna Yadavalli, Siva Kumar Nadimpalli

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Mammalian mannose 6-phosphate receptors (MPR 300 and 46) mediate transport of lysosomal enzymes to lysosomes. Recent studies established that the receptors are conserved throughout vertebrates. Although we purified the mollusc receptors and identified only a lysosomal enzyme receptor protein (LERP) in the Drosophila melanogaster, little is known about their structure and functional roles in the invertebrates. In the present study, we purified the putative receptors from the highly evolved invertebrate, starfish, cloned the cDNA for the MPR 46, and expressed it in mpr(-/-) mouse embryonic fibroblast cells. Structural comparison of starfish receptor sequences with other vertebrate receptors gave valuable information on its extensive structural homology with the vertebrate MPR 46 proteins. The expressed protein efficiently sorts lysosomal enzymes within the cells establishing a functional role for this protein. This first report on the invertebrate MPR 46 further confirms the structural and functional conservation of the receptor not only in the vertebrates but also in the invertebrates.

Original languageEnglish (US)
Pages (from-to)889-901
Number of pages13
JournalGlycoconjugate Journal
Volume25
Issue number9
DOIs
StatePublished - Dec 1 2008

Keywords

  • Echinodermata
  • Evolution
  • Goat
  • Invertebrate
  • MPR proteins
  • Mannose 6-phosphate receptors
  • Phosphomannan gel
  • Starfish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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