Many human L1 elements are capable of retrotransposition

Donna M. Sassaman, Beth A. Dombroski, John V. Moran, Michelle L. Kimberland, Thierry P. Naas, Ralph J. DeBerardinis, Abram Gabriel, Gary D. Swergold, Haig H. Kazazian

Research output: Contribution to journalArticle

313 Citations (Scopus)

Abstract

Using a selective screening strategy to enrich for active L1 elements, we isolated 13 full-length elements from a human genomic library. We tested these and two previously-isolated L1s (L1.3 and L1.4) for reverse transcriptase (RT) activity and the ability to retrotranspose in HeLa cells. Of the 13 newly-isolated L1s, eight had RT activity and three were able to retrotranspose. L1.3 and L1.4 possessed RT activity and retrotransposed at remarkably high frequencies. These studies bring the number of characterized active human L1 elements to seven. Based on these and other data, we estimate that 30-60 active L1 elements reside in the average diploid genome.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalNature Genetics
Volume16
Issue number1
StatePublished - May 1997

Fingerprint

Long Interspersed Nucleotide Elements
RNA-Directed DNA Polymerase
Genomic Library
Diploidy
HeLa Cells
Genome

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Sassaman, D. M., Dombroski, B. A., Moran, J. V., Kimberland, M. L., Naas, T. P., DeBerardinis, R. J., ... Kazazian, H. H. (1997). Many human L1 elements are capable of retrotransposition. Nature Genetics, 16(1), 37-43.

Many human L1 elements are capable of retrotransposition. / Sassaman, Donna M.; Dombroski, Beth A.; Moran, John V.; Kimberland, Michelle L.; Naas, Thierry P.; DeBerardinis, Ralph J.; Gabriel, Abram; Swergold, Gary D.; Kazazian, Haig H.

In: Nature Genetics, Vol. 16, No. 1, 05.1997, p. 37-43.

Research output: Contribution to journalArticle

Sassaman, DM, Dombroski, BA, Moran, JV, Kimberland, ML, Naas, TP, DeBerardinis, RJ, Gabriel, A, Swergold, GD & Kazazian, HH 1997, 'Many human L1 elements are capable of retrotransposition', Nature Genetics, vol. 16, no. 1, pp. 37-43.
Sassaman DM, Dombroski BA, Moran JV, Kimberland ML, Naas TP, DeBerardinis RJ et al. Many human L1 elements are capable of retrotransposition. Nature Genetics. 1997 May;16(1):37-43.
Sassaman, Donna M. ; Dombroski, Beth A. ; Moran, John V. ; Kimberland, Michelle L. ; Naas, Thierry P. ; DeBerardinis, Ralph J. ; Gabriel, Abram ; Swergold, Gary D. ; Kazazian, Haig H. / Many human L1 elements are capable of retrotransposition. In: Nature Genetics. 1997 ; Vol. 16, No. 1. pp. 37-43.
@article{d40189747d4040f985b5a8f6042cf750,
title = "Many human L1 elements are capable of retrotransposition",
abstract = "Using a selective screening strategy to enrich for active L1 elements, we isolated 13 full-length elements from a human genomic library. We tested these and two previously-isolated L1s (L1.3 and L1.4) for reverse transcriptase (RT) activity and the ability to retrotranspose in HeLa cells. Of the 13 newly-isolated L1s, eight had RT activity and three were able to retrotranspose. L1.3 and L1.4 possessed RT activity and retrotransposed at remarkably high frequencies. These studies bring the number of characterized active human L1 elements to seven. Based on these and other data, we estimate that 30-60 active L1 elements reside in the average diploid genome.",
author = "Sassaman, {Donna M.} and Dombroski, {Beth A.} and Moran, {John V.} and Kimberland, {Michelle L.} and Naas, {Thierry P.} and DeBerardinis, {Ralph J.} and Abram Gabriel and Swergold, {Gary D.} and Kazazian, {Haig H.}",
year = "1997",
month = "5",
language = "English (US)",
volume = "16",
pages = "37--43",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Many human L1 elements are capable of retrotransposition

AU - Sassaman, Donna M.

AU - Dombroski, Beth A.

AU - Moran, John V.

AU - Kimberland, Michelle L.

AU - Naas, Thierry P.

AU - DeBerardinis, Ralph J.

AU - Gabriel, Abram

AU - Swergold, Gary D.

AU - Kazazian, Haig H.

PY - 1997/5

Y1 - 1997/5

N2 - Using a selective screening strategy to enrich for active L1 elements, we isolated 13 full-length elements from a human genomic library. We tested these and two previously-isolated L1s (L1.3 and L1.4) for reverse transcriptase (RT) activity and the ability to retrotranspose in HeLa cells. Of the 13 newly-isolated L1s, eight had RT activity and three were able to retrotranspose. L1.3 and L1.4 possessed RT activity and retrotransposed at remarkably high frequencies. These studies bring the number of characterized active human L1 elements to seven. Based on these and other data, we estimate that 30-60 active L1 elements reside in the average diploid genome.

AB - Using a selective screening strategy to enrich for active L1 elements, we isolated 13 full-length elements from a human genomic library. We tested these and two previously-isolated L1s (L1.3 and L1.4) for reverse transcriptase (RT) activity and the ability to retrotranspose in HeLa cells. Of the 13 newly-isolated L1s, eight had RT activity and three were able to retrotranspose. L1.3 and L1.4 possessed RT activity and retrotransposed at remarkably high frequencies. These studies bring the number of characterized active human L1 elements to seven. Based on these and other data, we estimate that 30-60 active L1 elements reside in the average diploid genome.

UR - http://www.scopus.com/inward/record.url?scp=0030903621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030903621&partnerID=8YFLogxK

M3 - Article

C2 - 9140393

AN - SCOPUS:0030903621

VL - 16

SP - 37

EP - 43

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 1

ER -