Many human L1 elements are capable of retrotransposition

Donna M. Sassaman, Beth A. Dombroski, John V. Moran, Michelle L. Kimberland, Thierry P. Naas, Ralph J. DeBerardinis, Abram Gabriel, Gary D. Swergold, Haig H. Kazazian

Research output: Contribution to journalArticle

322 Scopus citations

Abstract

Using a selective screening strategy to enrich for active L1 elements, we isolated 13 full-length elements from a human genomic library. We tested these and two previously-isolated L1s (L1.3 and L1.4) for reverse transcriptase (RT) activity and the ability to retrotranspose in HeLa cells. Of the 13 newly-isolated L1s, eight had RT activity and three were able to retrotranspose. L1.3 and L1.4 possessed RT activity and retrotransposed at remarkably high frequencies. These studies bring the number of characterized active human L1 elements to seven. Based on these and other data, we estimate that 30-60 active L1 elements reside in the average diploid genome.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalNature genetics
Volume16
Issue number1
DOIs
StatePublished - May 1997

ASJC Scopus subject areas

  • Genetics

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