Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla

Changho Choi, Nicholas J. Coupland, Paramjit P. Bhardwaj, Nikolai Malykhin, Dan Gheorghiu, Peter S. Allen

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A new single-voxel proton NMR spectrally-selective refocusing method for measuring glutamate (Glu) and glutamine (Gln) in the human brain in vivo at 3T is reported. Triple-resonance selective 180° RF pulses with a bandwidth of 12 Hz were implemented within point-resolved spectroscopy (PRESS) for selective detection of Glu or Gln, and simultaneous acquisition of creatine singlets for use as a reference in phase correction. The carriers of the spectrally-selective 180° pulses and the echo times (TEs) were optimized with both numerical and experimental analyses of the filtering performance, which enabled measurements of the target metabolites with negligible contamination from N-acetylaspartate and glutathione. The concentrations of Glu and Gln in the prefrontal cortex were estimated to be 9.7 ± 0.5 and 3.0 ± 0.7 mM (mean ± SD, N = 7), with reference to Cr at 8 mM.

Original languageEnglish (US)
Pages (from-to)997-1005
Number of pages9
JournalMagnetic resonance in medicine
Volume55
Issue number5
DOIs
StatePublished - May 1 2006

Keywords

  • 3T
  • Glutamate
  • Glutamine
  • Human brain
  • Prefrontal cortex
  • RF pulse
  • Spectrally-selective refocusing
  • Triple-resonance selective 180°

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Choi, C., Coupland, N. J., Bhardwaj, P. P., Malykhin, N., Gheorghiu, D., & Allen, P. S. (2006). Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla. Magnetic resonance in medicine, 55(5), 997-1005. https://doi.org/10.1002/mrm.20875