Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid

Patricia M Jones, Richard Boriack, Eduard A. Struys, Dinesh Rakheja

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages16
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • 2-Hydroxyglutaric acid
  • 2HG
  • Biomarker
  • D-2-Hydroxyglutaric acid
  • D-2HG
  • L-2-Hydroxyglutaric acid
  • L-2HG
  • LC-MS/MS
  • Liquid chromatography-tandem mass spectrometry
  • Oncometabolite

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jones, P. M., Boriack, R., Struys, E. A., & Rakheja, D. (2017). Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. In Methods in Molecular Biology (Vol. 1633, pp. 219-234). (Methods in Molecular Biology; Vol. 1633). Humana Press Inc..