Mechanical Tension Drives Cell Membrane Fusion

Ji Hoon Kim, Yixin Ren, Win Pin Ng, Shuo Li, Sungmin Son, Yee Seir Kee, Shiliang Zhang, Guofeng Zhang, Daniel A. Fletcher, Douglas N. Robinson, Elizabeth Chen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Membrane fusion is an energy-consuming process that requires tight juxtaposition of two lipid bilayers. Little is known about how cells overcome energy barriers to bring their membranes together for fusion. Previously, we have shown that cell-cell fusion is an asymmetric process in which an "attacking" cell drills finger-like protrusions into the "receiving" cell to promote cell fusion. Here, we show that the receiving cell mounts a Myosin II (MyoII)-mediated mechanosensory response to its invasive fusion partner. MyoII acts as a mechanosensor, which directs its force-induced recruitment to the fusion site, and the mechanosensory response of MyoII is amplified by chemical signaling initiated by cell adhesion molecules. The accumulated MyoII, in turn, increases cortical tension and promotes fusion pore formation. We propose that the protrusive and resisting forces from fusion partners put the fusogenic synapse under high mechanical tension, which helps to overcome energy barriers for membrane apposition and drives cell membrane fusion.

Original languageEnglish (US)
Pages (from-to)561-573
Number of pages13
JournalDevelopmental Cell
Volume32
Issue number5
DOIs
StatePublished - Jan 1 2015

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Membrane Fusion
Cell Fusion
Myosin Type II
Cell membranes
Fusion reactions
Cell Membrane
Energy barriers
Mandrillus
Membranes
Cell Adhesion Molecules
Lipid Bilayers
Synapses
Lipid bilayers

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Kim, J. H., Ren, Y., Ng, W. P., Li, S., Son, S., Kee, Y. S., ... Chen, E. (2015). Mechanical Tension Drives Cell Membrane Fusion. Developmental Cell, 32(5), 561-573. https://doi.org/10.1016/j.devcel.2015.01.005

Mechanical Tension Drives Cell Membrane Fusion. / Kim, Ji Hoon; Ren, Yixin; Ng, Win Pin; Li, Shuo; Son, Sungmin; Kee, Yee Seir; Zhang, Shiliang; Zhang, Guofeng; Fletcher, Daniel A.; Robinson, Douglas N.; Chen, Elizabeth.

In: Developmental Cell, Vol. 32, No. 5, 01.01.2015, p. 561-573.

Research output: Contribution to journalArticle

Kim, JH, Ren, Y, Ng, WP, Li, S, Son, S, Kee, YS, Zhang, S, Zhang, G, Fletcher, DA, Robinson, DN & Chen, E 2015, 'Mechanical Tension Drives Cell Membrane Fusion', Developmental Cell, vol. 32, no. 5, pp. 561-573. https://doi.org/10.1016/j.devcel.2015.01.005
Kim JH, Ren Y, Ng WP, Li S, Son S, Kee YS et al. Mechanical Tension Drives Cell Membrane Fusion. Developmental Cell. 2015 Jan 1;32(5):561-573. https://doi.org/10.1016/j.devcel.2015.01.005
Kim, Ji Hoon ; Ren, Yixin ; Ng, Win Pin ; Li, Shuo ; Son, Sungmin ; Kee, Yee Seir ; Zhang, Shiliang ; Zhang, Guofeng ; Fletcher, Daniel A. ; Robinson, Douglas N. ; Chen, Elizabeth. / Mechanical Tension Drives Cell Membrane Fusion. In: Developmental Cell. 2015 ; Vol. 32, No. 5. pp. 561-573.
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