Mechanism for fatty acid "sparing" effect on glucose-induced transcription: Regulation of carbohydrate-responsive element-binding protein by AMP-activated protein kinase

Takumi Kawaguchi, Kiyoshi Osatomi, Hiromi Yamashita, Tsutomu Kabashima, Kosaku Uyeda

Research output: Contribution to journalArticle

309 Citations (Scopus)

Abstract

Carbohydrate-responsive element-binding protein (ChREBP) is a new transcription factor that binds to the carbohydrate-responsive element of the L-type pyruvate kinase gene (L-PK). The aim of this study was to investigate the mechanism by which feeding high fat diets results in decreased activity of ChREBP in the liver (Yamashita, H., Takenoshita, M., Sakurai, M., Bruick, R. K., Henzel, W. J., Shillinglaw, W., Arnot, D., and Uyeda, K. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 9116-9121). We cloned the rat liver ChREBP gene for use throughout this study. Acetate, octanoate, and palmitate inhibited the glucose-induced activation of L-PK transcription in ChREBP-overexpressed hepatocytes. In these hepatocytes, the cytosolic AMP concentration increased 30-fold and AMP-activated protein kinase activity was activated 2-fold. Similarly to the fatty acids, 5-amino-4-imidazolecarboxamide ribotide, a specific activator of AMP-activated protein kinase (AMPK) also inhibited the L-PK transcription activity in ChREBP-overexpressed hepatocytes. Using as a substrate a truncated ChREBP consisting of the C-terminal region, we demonstrated that phosphorylation by AMPK resulted in inactivation of the DNA binding activity. AMPK specifically phosphorylated Ser568 of ChREBP. A S568A mutant of the ChREBP gene showed tight DNA binding and lost its fatty acid sensitivity, whereas a S568D mutant showed weak DNA binding and inhibited L-PK transcription activity even in the absence of fatty acid. These results strongly suggested that the fatty acid inhibition of glucose-induced L-PK transcription resulted from AMPK phosphorylation of ChREBP at Ser568, which inactivated the DNA binding activity. AMPK was activated by the increased AMP that was generated by the fatty acid activation.

Original languageEnglish (US)
Pages (from-to)3829-3835
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number6
DOIs
StatePublished - Feb 8 2002

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AMP-Activated Protein Kinases
Transcription
Carrier Proteins
Fatty Acids
Carbohydrates
Glucose
Pyruvate Kinase
Genes
Hepatocytes
Phosphorylation
DNA
Adenosine Monophosphate
Liver
Chemical activation
Palmitates
High Fat Diet
Nutrition
Rats
Acetates
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mechanism for fatty acid "sparing" effect on glucose-induced transcription : Regulation of carbohydrate-responsive element-binding protein by AMP-activated protein kinase. / Kawaguchi, Takumi; Osatomi, Kiyoshi; Yamashita, Hiromi; Kabashima, Tsutomu; Uyeda, Kosaku.

In: Journal of Biological Chemistry, Vol. 277, No. 6, 08.02.2002, p. 3829-3835.

Research output: Contribution to journalArticle

Kawaguchi, Takumi ; Osatomi, Kiyoshi ; Yamashita, Hiromi ; Kabashima, Tsutomu ; Uyeda, Kosaku. / Mechanism for fatty acid "sparing" effect on glucose-induced transcription : Regulation of carbohydrate-responsive element-binding protein by AMP-activated protein kinase. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 6. pp. 3829-3835.
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