Mechanism of action of atpenin b on raji cells

K. Oshino, H. Kumagai, H. Tomoda, S. Omura

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Abstract

Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13∼0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5′-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting the inhibition of ATP-generating system by atpenin B.

Original languageEnglish (US)
Pages (from-to)1064-1068
Number of pages5
JournalThe Journal of Antibiotics
Volume43
Issue number9
DOIs
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Oshino, K., Kumagai, H., Tomoda, H., & Omura, S. (1990). Mechanism of action of atpenin b on raji cells. The Journal of Antibiotics, 43(9), 1064-1068. https://doi.org/10.7164/antibiotics.43.1064