Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13∼0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5′-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting the inhibition of ATP-generating system by atpenin B.
ASJC Scopus subject areas
- Drug Discovery