Mechanism of action of atpenin B on Raji cells

K. Oshino, H. Kumagai, H. Tomoda, S. Omura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13 ~ 0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5'-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting that inhibition of ATP-generating system by atpenin B.

Original languageEnglish (US)
Pages (from-to)1064-1068
Number of pages5
JournalJournal of Antibiotics
Volume43
Issue number9
StatePublished - 1990

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Inhibitory Concentration 50
Adenosine Triphosphate
Antifungal Agents
Palmitates
Penicillium
Leucine
Thymidine
atpenin B
Lipids
Growth

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Oshino, K., Kumagai, H., Tomoda, H., & Omura, S. (1990). Mechanism of action of atpenin B on Raji cells. Journal of Antibiotics, 43(9), 1064-1068.

Mechanism of action of atpenin B on Raji cells. / Oshino, K.; Kumagai, H.; Tomoda, H.; Omura, S.

In: Journal of Antibiotics, Vol. 43, No. 9, 1990, p. 1064-1068.

Research output: Contribution to journalArticle

Oshino, K, Kumagai, H, Tomoda, H & Omura, S 1990, 'Mechanism of action of atpenin B on Raji cells', Journal of Antibiotics, vol. 43, no. 9, pp. 1064-1068.
Oshino K, Kumagai H, Tomoda H, Omura S. Mechanism of action of atpenin B on Raji cells. Journal of Antibiotics. 1990;43(9):1064-1068.
Oshino, K. ; Kumagai, H. ; Tomoda, H. ; Omura, S. / Mechanism of action of atpenin B on Raji cells. In: Journal of Antibiotics. 1990 ; Vol. 43, No. 9. pp. 1064-1068.
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