Meibomianitis

Polar lipid abnormalities

Ward E. Shine, James P. McCulley

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Purpose: To determine abnormal meibomian gland secretion polar lipids from meibomianitis patients and to determine the significance of these lipid differences. Methods: Meibomian gland secretions were collected from controls and patients with chronic blepharitis. These meibomian gland lipids (meibum) were first separated by thin-layer chromatography. Polar lipids were then separated utilizing high-pressure liquid chromatography with ultraviolet detection. Individual peaks were identified by comparison to standards and retention time. Results: A polar lipid with an HPLC retention time longer than either phosphatidylcholine or sphingomyelin and shorter than phosphatidylethanolamine was significantly greater in the meibomianitis group (P < 0.05) compared with other chronic blepharitis groups and normals. This polar lipid's retention time was similar to that of dimethylphosphatidylcholine or possibly lysophosphatidylcholine, Additionally, meibomianitis polar lipids' normal fatty acids were highly unsaturated and differed from those of other groups, none of which were unsaturated. Conclusion: Many of the most significant differences in meibomianitis meibum composition occur in the polar lipids rather than the nonpolar lipids. Further investigations will determine the true significance of these findings.

Original languageEnglish (US)
Pages (from-to)781-783
Number of pages3
JournalCornea
Volume23
Issue number8
DOIs
StatePublished - Nov 2004

Fingerprint

Lipids
Meibomian Glands
Blepharitis
High Pressure Liquid Chromatography
Lysophosphatidylcholines
Sphingomyelins
Thin Layer Chromatography
Phosphatidylcholines
Fatty Acids

Keywords

  • Blepharitis
  • Lipids
  • Meibomianitis
  • Polar
  • Unsaturation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Meibomianitis : Polar lipid abnormalities. / Shine, Ward E.; McCulley, James P.

In: Cornea, Vol. 23, No. 8, 11.2004, p. 781-783.

Research output: Contribution to journalArticle

Shine, Ward E. ; McCulley, James P. / Meibomianitis : Polar lipid abnormalities. In: Cornea. 2004 ; Vol. 23, No. 8. pp. 781-783.
@article{aa2a81999b9a45d6a9af5bda03743904,
title = "Meibomianitis: Polar lipid abnormalities",
abstract = "Purpose: To determine abnormal meibomian gland secretion polar lipids from meibomianitis patients and to determine the significance of these lipid differences. Methods: Meibomian gland secretions were collected from controls and patients with chronic blepharitis. These meibomian gland lipids (meibum) were first separated by thin-layer chromatography. Polar lipids were then separated utilizing high-pressure liquid chromatography with ultraviolet detection. Individual peaks were identified by comparison to standards and retention time. Results: A polar lipid with an HPLC retention time longer than either phosphatidylcholine or sphingomyelin and shorter than phosphatidylethanolamine was significantly greater in the meibomianitis group (P < 0.05) compared with other chronic blepharitis groups and normals. This polar lipid's retention time was similar to that of dimethylphosphatidylcholine or possibly lysophosphatidylcholine, Additionally, meibomianitis polar lipids' normal fatty acids were highly unsaturated and differed from those of other groups, none of which were unsaturated. Conclusion: Many of the most significant differences in meibomianitis meibum composition occur in the polar lipids rather than the nonpolar lipids. Further investigations will determine the true significance of these findings.",
keywords = "Blepharitis, Lipids, Meibomianitis, Polar, Unsaturation",
author = "Shine, {Ward E.} and McCulley, {James P.}",
year = "2004",
month = "11",
doi = "10.1097/01.ico.0000133995.99520.1f",
language = "English (US)",
volume = "23",
pages = "781--783",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "8",

}

TY - JOUR

T1 - Meibomianitis

T2 - Polar lipid abnormalities

AU - Shine, Ward E.

AU - McCulley, James P.

PY - 2004/11

Y1 - 2004/11

N2 - Purpose: To determine abnormal meibomian gland secretion polar lipids from meibomianitis patients and to determine the significance of these lipid differences. Methods: Meibomian gland secretions were collected from controls and patients with chronic blepharitis. These meibomian gland lipids (meibum) were first separated by thin-layer chromatography. Polar lipids were then separated utilizing high-pressure liquid chromatography with ultraviolet detection. Individual peaks were identified by comparison to standards and retention time. Results: A polar lipid with an HPLC retention time longer than either phosphatidylcholine or sphingomyelin and shorter than phosphatidylethanolamine was significantly greater in the meibomianitis group (P < 0.05) compared with other chronic blepharitis groups and normals. This polar lipid's retention time was similar to that of dimethylphosphatidylcholine or possibly lysophosphatidylcholine, Additionally, meibomianitis polar lipids' normal fatty acids were highly unsaturated and differed from those of other groups, none of which were unsaturated. Conclusion: Many of the most significant differences in meibomianitis meibum composition occur in the polar lipids rather than the nonpolar lipids. Further investigations will determine the true significance of these findings.

AB - Purpose: To determine abnormal meibomian gland secretion polar lipids from meibomianitis patients and to determine the significance of these lipid differences. Methods: Meibomian gland secretions were collected from controls and patients with chronic blepharitis. These meibomian gland lipids (meibum) were first separated by thin-layer chromatography. Polar lipids were then separated utilizing high-pressure liquid chromatography with ultraviolet detection. Individual peaks were identified by comparison to standards and retention time. Results: A polar lipid with an HPLC retention time longer than either phosphatidylcholine or sphingomyelin and shorter than phosphatidylethanolamine was significantly greater in the meibomianitis group (P < 0.05) compared with other chronic blepharitis groups and normals. This polar lipid's retention time was similar to that of dimethylphosphatidylcholine or possibly lysophosphatidylcholine, Additionally, meibomianitis polar lipids' normal fatty acids were highly unsaturated and differed from those of other groups, none of which were unsaturated. Conclusion: Many of the most significant differences in meibomianitis meibum composition occur in the polar lipids rather than the nonpolar lipids. Further investigations will determine the true significance of these findings.

KW - Blepharitis

KW - Lipids

KW - Meibomianitis

KW - Polar

KW - Unsaturation

UR - http://www.scopus.com/inward/record.url?scp=7444264036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7444264036&partnerID=8YFLogxK

U2 - 10.1097/01.ico.0000133995.99520.1f

DO - 10.1097/01.ico.0000133995.99520.1f

M3 - Article

VL - 23

SP - 781

EP - 783

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 8

ER -