Membrane fission by dynamin

what we know and what we need to know

Bruno Antonny, Christopher Burd, Pietro De Camilli, Elizabeth Chen, Oliver Daumke, Katja Faelber, Marijn Ford, Vadim A. Frolov, Adam Frost, Jenny E. Hinshaw, Tom Kirchhausen, Michael M. Kozlov, Martin Lenz, Harry H. Low, Harvey McMahon, Christien Merrifield, Thomas D. Pollard, Phillip J. Robinson, Aurélien Roux, Sandra L Schmid

Research output: Contribution to journalReview article

132 Citations (Scopus)

Abstract

The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.

Original languageEnglish (US)
Pages (from-to)2270-2284
Number of pages15
JournalEMBO Journal
Volume35
Issue number21
DOIs
StatePublished - Nov 2 2016

Fingerprint

Dynamins
Membranes
Guanosine Triphosphate
GTP Phosphohydrolases
Muscle Contraction
Endocytosis
Oligomers
Synaptic Transmission
Organelles
Muscle
Hydrolysis
Polymers
Proteins
Fusion reactions

Keywords

  • dynamin
  • endocytosis
  • GTPase
  • membrane fission
  • molecular motor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Membrane fission by dynamin : what we know and what we need to know. / Antonny, Bruno; Burd, Christopher; De Camilli, Pietro; Chen, Elizabeth; Daumke, Oliver; Faelber, Katja; Ford, Marijn; Frolov, Vadim A.; Frost, Adam; Hinshaw, Jenny E.; Kirchhausen, Tom; Kozlov, Michael M.; Lenz, Martin; Low, Harry H.; McMahon, Harvey; Merrifield, Christien; Pollard, Thomas D.; Robinson, Phillip J.; Roux, Aurélien; Schmid, Sandra L.

In: EMBO Journal, Vol. 35, No. 21, 02.11.2016, p. 2270-2284.

Research output: Contribution to journalReview article

Antonny, B, Burd, C, De Camilli, P, Chen, E, Daumke, O, Faelber, K, Ford, M, Frolov, VA, Frost, A, Hinshaw, JE, Kirchhausen, T, Kozlov, MM, Lenz, M, Low, HH, McMahon, H, Merrifield, C, Pollard, TD, Robinson, PJ, Roux, A & Schmid, SL 2016, 'Membrane fission by dynamin: what we know and what we need to know', EMBO Journal, vol. 35, no. 21, pp. 2270-2284. https://doi.org/10.15252/embj.201694613
Antonny B, Burd C, De Camilli P, Chen E, Daumke O, Faelber K et al. Membrane fission by dynamin: what we know and what we need to know. EMBO Journal. 2016 Nov 2;35(21):2270-2284. https://doi.org/10.15252/embj.201694613
Antonny, Bruno ; Burd, Christopher ; De Camilli, Pietro ; Chen, Elizabeth ; Daumke, Oliver ; Faelber, Katja ; Ford, Marijn ; Frolov, Vadim A. ; Frost, Adam ; Hinshaw, Jenny E. ; Kirchhausen, Tom ; Kozlov, Michael M. ; Lenz, Martin ; Low, Harry H. ; McMahon, Harvey ; Merrifield, Christien ; Pollard, Thomas D. ; Robinson, Phillip J. ; Roux, Aurélien ; Schmid, Sandra L. / Membrane fission by dynamin : what we know and what we need to know. In: EMBO Journal. 2016 ; Vol. 35, No. 21. pp. 2270-2284.
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