Metabolic flux analysis of cellular growth using additional elemental composition data

Hongwu Ma, Xueming Zhao, Xiaofeng Guo, Jin Lu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Metabolic flux analysis of cellula growth is very important for the fermentation processes which have a small yield of metabolic products. A new analysis method is developed to calculate the least square solution of the intercellular metabolic flux distribution, which successfully combines cellular elemental composition data with cellular molecular composition data by incorporating carbon balance and generalized degree of reduction balance into the calculating process. This method can make the result more reliable, because it utilizes more measurement data and ensures data consistency. It should be mentioned that all results are derived from a simplified metabolic network of B. subtilis, which is created by representing the complex biomass synthesis network with some precursor requirement rates.

Original languageEnglish (US)
Pages (from-to)847-852
Number of pages6
JournalHuagong Xuebao/Journal of Chemical Industry and Engineering (China)
Volume53
Issue number8
StatePublished - Aug 1 2002

Keywords

  • B. subtilis
  • Cell composition
  • Cell growth process
  • Metabolic flux analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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