Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate

Jae Mo Park, Sonal Josan, Taichang Jang, Milton Merchant, Yi Fen Yen, Ralph E. Hurd, Lawrence Recht, Daniel M. Spielman, Dirk Mayer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In addition to an increased lactate-to-pyruvate ratio, altered metabolism of a malignant glioma can be further characterized by its kinetics. Spatially resolved dynamic data of pyruvate and lactate from C6-implanted female Sprague-Dawley rat brain were acquired using a spiral chemical shift imaging sequence after a bolus injection of a hyperpolarized [1-13C]pyruvate. Apparent rate constants for the conversion of pyruvate to lactate in three different regions (glioma, normal appearing brain, and vasculature) were estimated based on a two-site exchange model. The apparent conversion rate constant was 0.018 ± 0.004 s-1 (mean ± standard deviation, n = 6) for glioma, 0.009 ± 0.003 s-1 for normal brain, and 0.005 ± 0.001 s-1 for vasculature, whereas the lactate-to-pyruvate ratio, the metabolic marker used to date to identify tumor regions, was 0.36 ± 0.07 (mean ± SD), 0.24 ± 0.07, and 0.12 ± 0.02 for glioma, normal brain, and vasculature, respectively. The data suggest that the apparent conversion rate better differentiate glioma from normal brain (P = 0.001, n = 6) than the lactate-to-pyruvate ratio (P = 0.02).

Original languageEnglish (US)
Pages (from-to)1886-1893
Number of pages8
JournalMagnetic resonance in medicine
Volume68
Issue number6
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

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Pyruvic Acid
Glioma
Magnetic Resonance Imaging
Lactic Acid
Brain
Sprague Dawley Rats
Injections
Neoplasms

Keywords

  • glioma metabolism
  • hyperpolarized C
  • magnetic resonance spectroscopic imaging
  • metabolite kinetics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate. / Park, Jae Mo; Josan, Sonal; Jang, Taichang; Merchant, Milton; Yen, Yi Fen; Hurd, Ralph E.; Recht, Lawrence; Spielman, Daniel M.; Mayer, Dirk.

In: Magnetic resonance in medicine, Vol. 68, No. 6, 01.12.2012, p. 1886-1893.

Research output: Contribution to journalArticle

Park, JM, Josan, S, Jang, T, Merchant, M, Yen, YF, Hurd, RE, Recht, L, Spielman, DM & Mayer, D 2012, 'Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate', Magnetic resonance in medicine, vol. 68, no. 6, pp. 1886-1893. https://doi.org/10.1002/mrm.24181
Park, Jae Mo ; Josan, Sonal ; Jang, Taichang ; Merchant, Milton ; Yen, Yi Fen ; Hurd, Ralph E. ; Recht, Lawrence ; Spielman, Daniel M. ; Mayer, Dirk. / Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate. In: Magnetic resonance in medicine. 2012 ; Vol. 68, No. 6. pp. 1886-1893.
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abstract = "In addition to an increased lactate-to-pyruvate ratio, altered metabolism of a malignant glioma can be further characterized by its kinetics. Spatially resolved dynamic data of pyruvate and lactate from C6-implanted female Sprague-Dawley rat brain were acquired using a spiral chemical shift imaging sequence after a bolus injection of a hyperpolarized [1-13C]pyruvate. Apparent rate constants for the conversion of pyruvate to lactate in three different regions (glioma, normal appearing brain, and vasculature) were estimated based on a two-site exchange model. The apparent conversion rate constant was 0.018 ± 0.004 s-1 (mean ± standard deviation, n = 6) for glioma, 0.009 ± 0.003 s-1 for normal brain, and 0.005 ± 0.001 s-1 for vasculature, whereas the lactate-to-pyruvate ratio, the metabolic marker used to date to identify tumor regions, was 0.36 ± 0.07 (mean ± SD), 0.24 ± 0.07, and 0.12 ± 0.02 for glioma, normal brain, and vasculature, respectively. The data suggest that the apparent conversion rate better differentiate glioma from normal brain (P = 0.001, n = 6) than the lactate-to-pyruvate ratio (P = 0.02).",
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