Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate

Jae Mo Park, Sonal Josan, Taichang Jang, Milton Merchant, Yi Fen Yen, Ralph E. Hurd, Lawrence Recht, Daniel M. Spielman, Dirk Mayer

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

In addition to an increased lactate-to-pyruvate ratio, altered metabolism of a malignant glioma can be further characterized by its kinetics. Spatially resolved dynamic data of pyruvate and lactate from C6-implanted female Sprague-Dawley rat brain were acquired using a spiral chemical shift imaging sequence after a bolus injection of a hyperpolarized [1-13C]pyruvate. Apparent rate constants for the conversion of pyruvate to lactate in three different regions (glioma, normal appearing brain, and vasculature) were estimated based on a two-site exchange model. The apparent conversion rate constant was 0.018 ± 0.004 s-1 (mean ± standard deviation, n = 6) for glioma, 0.009 ± 0.003 s-1 for normal brain, and 0.005 ± 0.001 s-1 for vasculature, whereas the lactate-to-pyruvate ratio, the metabolic marker used to date to identify tumor regions, was 0.36 ± 0.07 (mean ± SD), 0.24 ± 0.07, and 0.12 ± 0.02 for glioma, normal brain, and vasculature, respectively. The data suggest that the apparent conversion rate better differentiate glioma from normal brain (P = 0.001, n = 6) than the lactate-to-pyruvate ratio (P = 0.02).

Original languageEnglish (US)
Pages (from-to)1886-1893
Number of pages8
JournalMagnetic resonance in medicine
Volume68
Issue number6
DOIs
StatePublished - Dec 1 2012

    Fingerprint

Keywords

  • glioma metabolism
  • hyperpolarized C
  • magnetic resonance spectroscopic imaging
  • metabolite kinetics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Park, J. M., Josan, S., Jang, T., Merchant, M., Yen, Y. F., Hurd, R. E., Recht, L., Spielman, D. M., & Mayer, D. (2012). Metabolite kinetics in C6 rat glioma model using magnetic resonance spectroscopic imaging of hyperpolarized [1-13C]pyruvate. Magnetic resonance in medicine, 68(6), 1886-1893. https://doi.org/10.1002/mrm.24181