Metallothionein expression is altered in a transgenic murine model of familial amyotrophic lateral sclerosis

Yun Hua Gong, Jeffrey L. Elliott

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Missense mutations in the gene encoding copper zinc superoxide dismutase (SOD1) have been found to cause one form of familial amyotrophic lateral sclerosis (FALS). Although the exact mechanism of disease is unknown, abnormalities in the ability of mutant SOD1 to bind zinc or copper ions may be crucial in the pathogenesis of disease. Because members of the metallothionein (MT) family of zinc and copper binding proteins function as important cellular regulators of metal ion bioavailability in the central nervous system, we used in situ hybridization and immunohistochemistry to study the expression pattern of these molecules in a transgenic mouse model of familial ALS. In adult wild-type mouse spinal cord, expression of MT-I and MT-II is restricted to ependymal cells and a subset of astrocytes located in white matter tracts, while MT-III synthesis is limited to neurons within gray matter. Compared to wild-type littermates, transgenic mice carrying the G93A SOD1 mutation demonstrate markedly increased expression of MT-I and MT-II within astrocytes in both white and gray matter as weakness develops. MT-III synthesis in neurons is also greatly upregulated as G93A SOD1 animals age, with glial cell expression of MT-III evident by later stages of the disease. Changes in MT expression occur before the onset of motor deficits or significant motor neuron pathology in G93A SOD1 mice and remarkably extend beyond ventral horn populations of neurons and glia. These results are consistent with the hypothesis that metallothioneins may serve an early and important protective function in FALS. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalExperimental Neurology
Volume162
Issue number1
DOIs
StatePublished - Mar 2000

Keywords

  • ALS
  • Astrocyte
  • Motor neuron
  • Superoxide dismutase
  • Zinc

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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