Metallothioneins protect cytosolic creatine kinases against stress induced by nitrogen-based oxidants

Zhe Chen, Jie Li, Tong Jin Zhao, Xu Hui Li, Fan Guo Meng, Hang Mu, Yong Bin Yan, Hai Meng Zhou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The formation of intracellular nitrogen-based oxidants has important physiological and pathological consequences. CK (creatine kinase), which plays a key role in intracellular energy metabolism, is a main target of low concentrations of oxidative and nitrative stresses. In the present study, the interaction between cytosolic CKs [MM-CK (muscle-type CK) and BB-CK (brain-type CK)] and MTs [metallothioneins; hMT2A (human MT-IIA) and hMT3 (human MT-III)] were characterized by both in vitro and intact-cell assays. MTs could successfully protect the cytosolic CKs against inactivation induced by low concentrations of PN (peroxynitrite) and NO both in vitro and in hMT2A-overexpressing H9c2 cells and hMT3-knockdown U-87 MG cells. Under high PN concentrations, CK formed granule-like structures, and MTs were well co-localized in these aggregated granules. Further analysis indicated that the number of cells containing the CK aggregates negatively correlated with the expression levels of MTs. In vitro experiments indicated that MTs could effectively protect CKs against aggregation during refolding, suggesting that MT might function as a chaperone to assist CK re-activation. The findings of the present study provide direct evidence of the connection between the two well-characterized intracellular systems: the precisely balanced energy homoeostasis by CKs and the oxidative-stress response system using MTs.

Original languageEnglish (US)
Pages (from-to)623-632
Number of pages10
JournalBiochemical Journal
Volume441
Issue number2
DOIs
StatePublished - Jan 15 2012

Fingerprint

Metallothionein
Creatine Kinase
Oxidants
Nitrogen
BB Form Creatine Kinase
MM Form Creatine Kinase
Peroxynitrous Acid
Oxidative Stress
Oxidative stress
Energy Metabolism
Assays
Homeostasis
Agglomeration
Cell Count
Chemical activation
In Vitro Techniques
Experiments

Keywords

  • Creatine kinase (CK)
  • Metallothionein (MT)
  • Molecular chaperone
  • Nitrative stress
  • Oxidative stress
  • Peroxynitrite (PN)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Chen, Z., Li, J., Zhao, T. J., Li, X. H., Meng, F. G., Mu, H., ... Zhou, H. M. (2012). Metallothioneins protect cytosolic creatine kinases against stress induced by nitrogen-based oxidants. Biochemical Journal, 441(2), 623-632. https://doi.org/10.1042/BJ20111565

Metallothioneins protect cytosolic creatine kinases against stress induced by nitrogen-based oxidants. / Chen, Zhe; Li, Jie; Zhao, Tong Jin; Li, Xu Hui; Meng, Fan Guo; Mu, Hang; Yan, Yong Bin; Zhou, Hai Meng.

In: Biochemical Journal, Vol. 441, No. 2, 15.01.2012, p. 623-632.

Research output: Contribution to journalArticle

Chen, Z, Li, J, Zhao, TJ, Li, XH, Meng, FG, Mu, H, Yan, YB & Zhou, HM 2012, 'Metallothioneins protect cytosolic creatine kinases against stress induced by nitrogen-based oxidants', Biochemical Journal, vol. 441, no. 2, pp. 623-632. https://doi.org/10.1042/BJ20111565
Chen, Zhe ; Li, Jie ; Zhao, Tong Jin ; Li, Xu Hui ; Meng, Fan Guo ; Mu, Hang ; Yan, Yong Bin ; Zhou, Hai Meng. / Metallothioneins protect cytosolic creatine kinases against stress induced by nitrogen-based oxidants. In: Biochemical Journal. 2012 ; Vol. 441, No. 2. pp. 623-632.
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