MHC Class I gene organization in >1.5-Mb YAC contigs from the H2-M region

Elsy P. Jones, Hong Xiao, Roger A. Schultz, Lorraine Flaherty, Zdenek Trachtulec, Vladimir Vincek, Zoia Larin, Hans Lehrach, Kirsten Fischer Lindahl

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single-and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A//M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.

Original languageEnglish (US)
Pages (from-to)40-51
Number of pages12
JournalGenomics
Volume27
Issue number1
DOIs
StatePublished - May 1 1995

ASJC Scopus subject areas

  • Genetics

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