MiR-155 suppresses bacterial clearance in Pseudomonas aeruginosa-induced keratitis by targeting Rheb

Kun Yang, Minhao Wu, Meiyu Li, Dandan Li, Anping Peng, Xinxin Nie, Mingxia Sun, Jinli Wang, Yongjian Wu, Qiuchan Deng, Min Zhu, Kang Chen, Jin Yuan, Xi Huang

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

miR-155 (microRNA-155) is an important noncoding RNA in regulating host inflammatory responses. However, its regulatory role in ocular infection remains unclear. Our study first explored the function of miR-155 in Pseudomonas aeruginosa-induced keratitis, one of the most common sight-threatening ocular diseases. We found that miR-155 expression was enhanced in human and mouse corneas after P. aeruginosa infection and was mainly expressed in macrophages but not neutrophils. In vivo studies demonstrated that miR-155 knockout mice displayed more resistance to P. aeruginosa keratitis, with a higher inducible nitric oxide synthase level and a lower bacterial burden. More importantly, in vitro data indicated that miR-155 suppressed the macrophage-mediated bacterial phagocytosis and intracellular killing of P. aeruginosa by targeting Rheb (Ras homolog enriched in brain). To the best of our knowledge, this is the first study to explore the role of miR-155 in bacterial keratitis, which may provide a promising target for clinical treatment of P. aeruginosa keratitis and other infectious diseases.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalJournal of Infectious Diseases
Volume210
Issue number1
DOIs
StatePublished - Jul 1 2014
Externally publishedYes

Keywords

  • Bacterial killing
  • Corneal infection
  • Inflammation
  • MiR-155
  • Phagocytosis
  • Pseudomonas aeruginosa
  • Rheb

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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