Modifying ligand specificity of gene regulatory proteins

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nuclear receptors contain a conserved hydrophobic ligand binding pocket that is particularly amenable to structure-based protein engineering. Thus, site-directed mutagenesis of the ligand binding pocket has resulted in the creation of nuclear receptors with novel ligand specificities. Such proteins are now being used to control gene expression in vivo in a ligand-dependent manner.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalCurrent Opinion in Chemical Biology
Volume4
Issue number1
DOIs
StatePublished - Feb 1 2000

Fingerprint

Genes
Ligands
Cytoplasmic and Nuclear Receptors
Proteins
Protein Engineering
Mutagenesis
Site-Directed Mutagenesis
Gene expression
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Modifying ligand specificity of gene regulatory proteins. / Doyle, Donald F.; Mangelsdorf, David J.; Corey, David R.

In: Current Opinion in Chemical Biology, Vol. 4, No. 1, 01.02.2000, p. 60-63.

Research output: Contribution to journalArticle

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