TY - JOUR
T1 - Modulation of assembly and disassembly of a new tetraphenylethene based nanosensor for highly selective detection of hyaluronidase
AU - Li, Xiangqian
AU - Zhou, Zhan
AU - Tang, Yiping
AU - Zhang, Chengcheng
AU - Zheng, Yuhui
AU - Gao, Jinwei
AU - Wang, Qianming
N1 - Funding Information:
Q. M. acknowledges the support from National Natural Science Foundation of China ( 21371063 ) and Guangdong Science and Technology plan ( 2016A050502053 ). Z. Z. acknowledges the Scientific Research Fund of Henan Provincial Education Department ( 17A150016 ) and Natural Science Foundation of Henan ( 162300410200 ) for financial assistances. Y. H. thanks Research fund of higher education committee of Guangdong province ( GDJ2016003 ). J.W. is grateful to the support from Innovation team project by department of Education of Guangdong Province ( 2016KCXTD009 ).
PY - 2018/12/10
Y1 - 2018/12/10
N2 - The development of nanoprobes with high sensitivity and specificity for tumor marker detection has gained increasing attention in biological applications. Here, we have designed and synthesized a novel 4,4’,4”,4”’-(ethene-1,1,2,2-tetrayltetrakis(benzene-4,1-diyl))tetrakis (1-(4-bromobenzyl)pyridin-1-ium) bromide (TPE-4N+) based aggregation induced emission (AIE) fluorescent sensor and it gives rise to electrostatic adsorption towards hyaluronic acid (HA), resulting in an effective emission recovery in yellow-greenish region. In the presence of hyaluronidase (HAase), the enzymatic digestion between HA and HAase induces the fluorescence quenching and this “on-off” change has been analyzed by two consecutive linear equations. The low detection limit is determined to be 0.02 U/mL by quantitative evaluation and its practical application has been verified by detecting human urine samples. It is promising that this new approach can be utilized to study a wide variety of other depolymerization reactions.
AB - The development of nanoprobes with high sensitivity and specificity for tumor marker detection has gained increasing attention in biological applications. Here, we have designed and synthesized a novel 4,4’,4”,4”’-(ethene-1,1,2,2-tetrayltetrakis(benzene-4,1-diyl))tetrakis (1-(4-bromobenzyl)pyridin-1-ium) bromide (TPE-4N+) based aggregation induced emission (AIE) fluorescent sensor and it gives rise to electrostatic adsorption towards hyaluronic acid (HA), resulting in an effective emission recovery in yellow-greenish region. In the presence of hyaluronidase (HAase), the enzymatic digestion between HA and HAase induces the fluorescence quenching and this “on-off” change has been analyzed by two consecutive linear equations. The low detection limit is determined to be 0.02 U/mL by quantitative evaluation and its practical application has been verified by detecting human urine samples. It is promising that this new approach can be utilized to study a wide variety of other depolymerization reactions.
KW - Aggregation induced emission
KW - Hyaluronidase
KW - Sensor
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U2 - 10.1016/j.snb.2018.08.093
DO - 10.1016/j.snb.2018.08.093
M3 - Article
AN - SCOPUS:85052065153
SN - 0925-4005
VL - 276
SP - 95
EP - 100
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -