Modulation of human natural killer cell function by L-leucine methyl ester: Monocyte-dependent depletion from human peripheral blood mononuclear cells

Dwain L Thiele, P. E. Lipsky

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Abstract

L-leucine methyl ester (Leu-OMe) causes lysosomal disruption and death of human monocytes (Mφ). In addition, Leu-OMe removed natural killer cell (NK) activity from human peripheral mononuclear cells (PBM). Thus, a brief preincubation of PBM with Leu-OMe (>1 mM) caused irreversible loss of NK function as assessed by the lysis of K562 targets. By contrast, a variety of other amino acid methyl esters, including L-glutamic dimethyl ester, L-valine methyl ester, and L-isoleucine methyl ester caused reversible inhibition of NK activity in a manner that was similar to other lysosomotropic agents such as chloroquine and ammonium chloride, but did not cause irreversible loss of all NK function. Leu-OMe appeared to cause actual removal of NK effector cells from PBM, because K562 target binding cells, Leu-11b+ lymphocytes, and OKM1+ lymphocytes were depleted. If Mφ were removed from PBM before the incubation, Leu-OMe caused only reversible inhibition of NK function in a manner similar to that observed with other amino acid methyl esters. Upon the addition of freshly isolated Mφ, polymorphonuclear leukocytes, or sonicates of these cells to Mφ-depleted lymphocyte populations, irreversible ablation of NK function was again observed as a result of Leu-OMe exposure. After in vitro culture, Mφ lost their susceptibility to Leu-OMe toxicity and the ability to mediate the irreversible deletion of NK cells resulting from Leu-OMe incubation. These results indicate that in the absence of Mφ, Leu-OMe and a variety of other amino acid methyl esters are reversible inhibitors of NK function. However, Leu-OMe is unique in that it can interact with Mφ or granulocytes to effect an irreversible loss of NK activity from human peripheral blood lymphocytes.

Original languageEnglish (US)
Pages (from-to)786-793
Number of pages8
JournalJournal of Immunology
Volume134
Issue number2
StatePublished - 1985

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Leucine
Natural Killer Cells
Monocytes
Blood Cells
Esters
Lymphocytes
Amino Acids
Human Activities
leucine methyl ester
Ammonium Chloride
Isoleucine
Chloroquine
Valine
Granulocytes
Neutrophils
phytobacteriomycin

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Modulation of human natural killer cell function by L-leucine methyl ester: Monocyte-dependent depletion from human peripheral blood mononuclear cells",
abstract = "L-leucine methyl ester (Leu-OMe) causes lysosomal disruption and death of human monocytes (Mφ). In addition, Leu-OMe removed natural killer cell (NK) activity from human peripheral mononuclear cells (PBM). Thus, a brief preincubation of PBM with Leu-OMe (>1 mM) caused irreversible loss of NK function as assessed by the lysis of K562 targets. By contrast, a variety of other amino acid methyl esters, including L-glutamic dimethyl ester, L-valine methyl ester, and L-isoleucine methyl ester caused reversible inhibition of NK activity in a manner that was similar to other lysosomotropic agents such as chloroquine and ammonium chloride, but did not cause irreversible loss of all NK function. Leu-OMe appeared to cause actual removal of NK effector cells from PBM, because K562 target binding cells, Leu-11b+ lymphocytes, and OKM1+ lymphocytes were depleted. If Mφ were removed from PBM before the incubation, Leu-OMe caused only reversible inhibition of NK function in a manner similar to that observed with other amino acid methyl esters. Upon the addition of freshly isolated Mφ, polymorphonuclear leukocytes, or sonicates of these cells to Mφ-depleted lymphocyte populations, irreversible ablation of NK function was again observed as a result of Leu-OMe exposure. After in vitro culture, Mφ lost their susceptibility to Leu-OMe toxicity and the ability to mediate the irreversible deletion of NK cells resulting from Leu-OMe incubation. These results indicate that in the absence of Mφ, Leu-OMe and a variety of other amino acid methyl esters are reversible inhibitors of NK function. However, Leu-OMe is unique in that it can interact with Mφ or granulocytes to effect an irreversible loss of NK activity from human peripheral blood lymphocytes.",
author = "Thiele, {Dwain L} and Lipsky, {P. E.}",
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T2 - Monocyte-dependent depletion from human peripheral blood mononuclear cells

AU - Thiele, Dwain L

AU - Lipsky, P. E.

PY - 1985

Y1 - 1985

N2 - L-leucine methyl ester (Leu-OMe) causes lysosomal disruption and death of human monocytes (Mφ). In addition, Leu-OMe removed natural killer cell (NK) activity from human peripheral mononuclear cells (PBM). Thus, a brief preincubation of PBM with Leu-OMe (>1 mM) caused irreversible loss of NK function as assessed by the lysis of K562 targets. By contrast, a variety of other amino acid methyl esters, including L-glutamic dimethyl ester, L-valine methyl ester, and L-isoleucine methyl ester caused reversible inhibition of NK activity in a manner that was similar to other lysosomotropic agents such as chloroquine and ammonium chloride, but did not cause irreversible loss of all NK function. Leu-OMe appeared to cause actual removal of NK effector cells from PBM, because K562 target binding cells, Leu-11b+ lymphocytes, and OKM1+ lymphocytes were depleted. If Mφ were removed from PBM before the incubation, Leu-OMe caused only reversible inhibition of NK function in a manner similar to that observed with other amino acid methyl esters. Upon the addition of freshly isolated Mφ, polymorphonuclear leukocytes, or sonicates of these cells to Mφ-depleted lymphocyte populations, irreversible ablation of NK function was again observed as a result of Leu-OMe exposure. After in vitro culture, Mφ lost their susceptibility to Leu-OMe toxicity and the ability to mediate the irreversible deletion of NK cells resulting from Leu-OMe incubation. These results indicate that in the absence of Mφ, Leu-OMe and a variety of other amino acid methyl esters are reversible inhibitors of NK function. However, Leu-OMe is unique in that it can interact with Mφ or granulocytes to effect an irreversible loss of NK activity from human peripheral blood lymphocytes.

AB - L-leucine methyl ester (Leu-OMe) causes lysosomal disruption and death of human monocytes (Mφ). In addition, Leu-OMe removed natural killer cell (NK) activity from human peripheral mononuclear cells (PBM). Thus, a brief preincubation of PBM with Leu-OMe (>1 mM) caused irreversible loss of NK function as assessed by the lysis of K562 targets. By contrast, a variety of other amino acid methyl esters, including L-glutamic dimethyl ester, L-valine methyl ester, and L-isoleucine methyl ester caused reversible inhibition of NK activity in a manner that was similar to other lysosomotropic agents such as chloroquine and ammonium chloride, but did not cause irreversible loss of all NK function. Leu-OMe appeared to cause actual removal of NK effector cells from PBM, because K562 target binding cells, Leu-11b+ lymphocytes, and OKM1+ lymphocytes were depleted. If Mφ were removed from PBM before the incubation, Leu-OMe caused only reversible inhibition of NK function in a manner similar to that observed with other amino acid methyl esters. Upon the addition of freshly isolated Mφ, polymorphonuclear leukocytes, or sonicates of these cells to Mφ-depleted lymphocyte populations, irreversible ablation of NK function was again observed as a result of Leu-OMe exposure. After in vitro culture, Mφ lost their susceptibility to Leu-OMe toxicity and the ability to mediate the irreversible deletion of NK cells resulting from Leu-OMe incubation. These results indicate that in the absence of Mφ, Leu-OMe and a variety of other amino acid methyl esters are reversible inhibitors of NK function. However, Leu-OMe is unique in that it can interact with Mφ or granulocytes to effect an irreversible loss of NK activity from human peripheral blood lymphocytes.

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