Molecular analysis of gamma-ray-induced mutations at the hprt locus in primary human skin fibroblasts by multiplex polymerase chain reaction

M. S. Park, T. Hanks, A. Jaberaboansari, D. J. Chen

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36 Scopus citations

Abstract

A total of 153 hprt mutants (23 spontaneous, 130 γ-ray-induced) of primary human skin fibroblasts were isolated and genetic alterations at the locus were studied by multiplex polymerase chain reaction (PCR). The analyses showed that 51% (66/130) of γ-ray-induced genetic changes were large deletions, whereas the majority of spontaneous mutants (21/23) exhibited point mutations. The spectrum of large genetic alterations appeared to be dependent on dose in γ-ray-induced (1-4 Gy) mutations; mutants with complete loss of the hprt locus comprised 21 (3/14) or 39% (15/38) of clones isolated after irradiation with 1 or 4 Gy, respectively. The frequency of partial deletions was found to be higher in the mutants isolated from clones irradiated with 2 Gy (38%) than from those irradiated with 4 Gy (8%). Mapping of all intragenic deletion breakpoints exhibited a nonrandom distribution of breakpoints toward the 3' end of the hprt gene.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalRadiation research
Volume141
Issue number1
DOIs
StatePublished - Mar 20 1995

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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