TY - JOUR
T1 - Molecular characterization of invasive Staphylococcus aureus infection in central New York children
T2 - Importance of two clonal groups and inconsistent presence of selected virulence determinants
AU - Suryadevara, Manika
AU - Clark, Andrew E.
AU - Wolk, Donna M.
AU - Carman, Aubri
AU - Rosenbaum, Paula F.
AU - Shaw, Jana
PY - 2013/3
Y1 - 2013/3
N2 - Background. The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods. Clinical and demographic information for hospitalized children <19 years of age with community-onset or community-associated ISA infections, determined from clinical microbiology records, was extracted from medical records from Upstate Golisano Children's Hospital in CNY. Antibiotic susceptibility was assessed, and available isolates were genotyped and tested for the presence of selected virulence determinants. Associations between clinical and laboratory findings were evaluated using standard statistical techniques. Results. Ninety patients with ISA disease diagnosed between 2007 and 2010 were included in the study; 74% were due to methicillin-susceptible SA (MSSA). The most common clinical diagnosis was bacteremia. Fifty-seven of 90 isolates were available for further testing. The SA pulsed-field gel electrophoresis type, agr type, and clonal complexes most commonly isolated were USA300 (n = 25, 44%), agr1 (n = 30, 52%), and CC8 (n = 25, 44%), respectively. USA300 strains were more likely to be associated with deep abscesses (P =.007), whereas non-USA300 strains were associated with medical device infections (P =.018). Isolates from patients with deep abscesses and pneumonia were more likely to carry luk-PV genes (P =.023 and P =.051, respectively). Conclusions. MSSA remains an important problem of pediatric ISA infection in our region and results from genetically diverse SA populations.
AB - Background. The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods. Clinical and demographic information for hospitalized children <19 years of age with community-onset or community-associated ISA infections, determined from clinical microbiology records, was extracted from medical records from Upstate Golisano Children's Hospital in CNY. Antibiotic susceptibility was assessed, and available isolates were genotyped and tested for the presence of selected virulence determinants. Associations between clinical and laboratory findings were evaluated using standard statistical techniques. Results. Ninety patients with ISA disease diagnosed between 2007 and 2010 were included in the study; 74% were due to methicillin-susceptible SA (MSSA). The most common clinical diagnosis was bacteremia. Fifty-seven of 90 isolates were available for further testing. The SA pulsed-field gel electrophoresis type, agr type, and clonal complexes most commonly isolated were USA300 (n = 25, 44%), agr1 (n = 30, 52%), and CC8 (n = 25, 44%), respectively. USA300 strains were more likely to be associated with deep abscesses (P =.007), whereas non-USA300 strains were associated with medical device infections (P =.018). Isolates from patients with deep abscesses and pneumonia were more likely to carry luk-PV genes (P =.023 and P =.051, respectively). Conclusions. MSSA remains an important problem of pediatric ISA infection in our region and results from genetically diverse SA populations.
KW - Children
KW - Invasive
KW - MRSA
KW - MSSA
KW - S aureus
KW - USA300
KW - Virulence determinants
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U2 - 10.1093/jpids/pis087
DO - 10.1093/jpids/pis087
M3 - Article
C2 - 26619440
AN - SCOPUS:84939205177
SN - 2048-7193
VL - 2
SP - 30
EP - 39
JO - Journal of the Pediatric Infectious Diseases Society
JF - Journal of the Pediatric Infectious Diseases Society
IS - 1
M1 - pis087
ER -