Molecular cloning of rat klotho cDNA: Markedly decreased expression of klotho by acute inflammatory stress

Yoshio Ohyama, Masahiko Kurabayashi, Hiroaki Masuda, Tetsuya Nakamura, Yasushi Aihara, Tadashi Kaname, Tatsuo Suga, Masashi Arai, Hiroki Aizawa, Yutaka Matsumura, Makoto Kuro-o, Yo ichi Nabeshima, Ryozo Nagail

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

We have recently identified a novel gene, termed klotho that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the β-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho.

Original languageEnglish (US)
Pages (from-to)920-925
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume251
Issue number3
DOIs
StatePublished - Oct 29 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ohyama, Y., Kurabayashi, M., Masuda, H., Nakamura, T., Aihara, Y., Kaname, T., Suga, T., Arai, M., Aizawa, H., Matsumura, Y., Kuro-o, M., Nabeshima, Y. I., & Nagail, R. (1998). Molecular cloning of rat klotho cDNA: Markedly decreased expression of klotho by acute inflammatory stress. Biochemical and Biophysical Research Communications, 251(3), 920-925. https://doi.org/10.1006/bbrc.1998.9576