TY - JOUR
T1 - Molecular cloning of rat klotho cDNA
T2 - Markedly decreased expression of klotho by acute inflammatory stress
AU - Ohyama, Yoshio
AU - Kurabayashi, Masahiko
AU - Masuda, Hiroaki
AU - Nakamura, Tetsuya
AU - Aihara, Yasushi
AU - Kaname, Tadashi
AU - Suga, Tatsuo
AU - Arai, Masashi
AU - Aizawa, Hiroki
AU - Matsumura, Yutaka
AU - Kuro-o, Makoto
AU - Nabeshima, Yo ichi
AU - Nagail, Ryozo
N1 - Funding Information:
We thank Ms. M. Yamazaki, Ms. K. Ishihara, Ms. S. Saiki, and Ms. T. Nonaka for their technical assistance. We also acknowledge Ms. A. Yoguchi for her secretarial assistance. This study was supported in part by research grants from the Japanese Ministry of Education, Science, and Culture and by the Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR) of Japan. This study was also supported in part by a Research Grant from the Tokyo Hypertension Conference.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/10/29
Y1 - 1998/10/29
N2 - We have recently identified a novel gene, termed klotho that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the β-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho.
AB - We have recently identified a novel gene, termed klotho that is involved in the suppression of several aging phenotypes. The gene encodes a membrane protein that shares sequence similarity with the β-glucosidases of bacteria and plants. In this study, we isolated rat klotho cDNA and examined its tissue distribution in rats. The deduced amino acid sequence of rat Klotho protein was 1014 amino acids in length and 94 and 85% homologous to those of mouse and human klotho proteins, respectively. Northern blot analysis using the rat klotho cDNA probe identified a single transcript of 5.2 kb in size expressed predominantly in the kidney, while RT-PCR detected low levels of expression also in the brain, lung, intestine, and ovaries. During development, klotho expression in the kidney was markedly augmented after birth. Chromosomal localization of rat klotho was mapped to 12q12. Northern blot analysis showed that expression of klotho was markedly decreased by lipopolysaccharide (LPS) in vivo, suggesting that expression of klotho is affected by acute inflammatory stress. The present study leads to a better understanding of the physiologic and pathophysiologic roles of Klotho.
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U2 - 10.1006/bbrc.1998.9576
DO - 10.1006/bbrc.1998.9576
M3 - Article
C2 - 9791011
AN - SCOPUS:0032578906
VL - 251
SP - 920
EP - 925
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -