Molecular diagnosis of oligodendroglioma in paraffin sections

Kimmo J. Hatanpaa, Peter C. Burger, James R. Eshleman, Kathleen M. Murphy, Karin D. Berg

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Distinction of oligodendrogliomas from other gliomas is clinically important, but the histologic diagnosis of oligodendroglioma has been a difficult and notoriously subjective task. Testing for loss of heterozygosity (LOH) on chromosomal arms 1p and 19q, the genetic signature of oligodendroglioma, has emerged as a useful, objective adjunct to the traditional histologic evaluation. However, LOH testing of glioma specimens has not yet been widely implemented, presumably because of a lack of a practical LOH assay. We describe a 1p and 19q LOH assay suitable for routine diagnostics. In contrast to traditional microsatellite-based LOH analysis, we show that detection of LOH is usually possible even without normal tissue or blood from the same patient. A small area of tumor on a single paraffin section is sufficient for the assay. The assay protocol consists of a one-step DNA extraction, multiplex PCR for microsatellites on 1p and 19q, and capillary electrophoresis of the PCR products. LOH is detected by analysis of the allelic patterns and by integration of data from multiple highly polymorphic microsatellites. In a validation study on 19 gliomas, the results were concordant with results obtained by established methods and correlated well with histologic diagnoses. Because only a paraffin section is required, the pathologist can perform both the traditional histopathologic evaluation and this supporting molecular assay from the material at hand.

Original languageEnglish (US)
Pages (from-to)419-428
Number of pages10
JournalLaboratory Investigation
Volume83
Issue number3
StatePublished - Mar 1 2003

Fingerprint

Oligodendroglioma
Loss of Heterozygosity
Paraffin
Glioma
Microsatellite Repeats
Validation Studies
Multiplex Polymerase Chain Reaction
Capillary Electrophoresis
Arm
Hand
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hatanpaa, K. J., Burger, P. C., Eshleman, J. R., Murphy, K. M., & Berg, K. D. (2003). Molecular diagnosis of oligodendroglioma in paraffin sections. Laboratory Investigation, 83(3), 419-428.

Molecular diagnosis of oligodendroglioma in paraffin sections. / Hatanpaa, Kimmo J.; Burger, Peter C.; Eshleman, James R.; Murphy, Kathleen M.; Berg, Karin D.

In: Laboratory Investigation, Vol. 83, No. 3, 01.03.2003, p. 419-428.

Research output: Contribution to journalArticle

Hatanpaa, KJ, Burger, PC, Eshleman, JR, Murphy, KM & Berg, KD 2003, 'Molecular diagnosis of oligodendroglioma in paraffin sections', Laboratory Investigation, vol. 83, no. 3, pp. 419-428.
Hatanpaa KJ, Burger PC, Eshleman JR, Murphy KM, Berg KD. Molecular diagnosis of oligodendroglioma in paraffin sections. Laboratory Investigation. 2003 Mar 1;83(3):419-428.
Hatanpaa, Kimmo J. ; Burger, Peter C. ; Eshleman, James R. ; Murphy, Kathleen M. ; Berg, Karin D. / Molecular diagnosis of oligodendroglioma in paraffin sections. In: Laboratory Investigation. 2003 ; Vol. 83, No. 3. pp. 419-428.
@article{13ed0ec30a99416d8c4a25b5de91b0d6,
title = "Molecular diagnosis of oligodendroglioma in paraffin sections",
abstract = "Distinction of oligodendrogliomas from other gliomas is clinically important, but the histologic diagnosis of oligodendroglioma has been a difficult and notoriously subjective task. Testing for loss of heterozygosity (LOH) on chromosomal arms 1p and 19q, the genetic signature of oligodendroglioma, has emerged as a useful, objective adjunct to the traditional histologic evaluation. However, LOH testing of glioma specimens has not yet been widely implemented, presumably because of a lack of a practical LOH assay. We describe a 1p and 19q LOH assay suitable for routine diagnostics. In contrast to traditional microsatellite-based LOH analysis, we show that detection of LOH is usually possible even without normal tissue or blood from the same patient. A small area of tumor on a single paraffin section is sufficient for the assay. The assay protocol consists of a one-step DNA extraction, multiplex PCR for microsatellites on 1p and 19q, and capillary electrophoresis of the PCR products. LOH is detected by analysis of the allelic patterns and by integration of data from multiple highly polymorphic microsatellites. In a validation study on 19 gliomas, the results were concordant with results obtained by established methods and correlated well with histologic diagnoses. Because only a paraffin section is required, the pathologist can perform both the traditional histopathologic evaluation and this supporting molecular assay from the material at hand.",
author = "Hatanpaa, {Kimmo J.} and Burger, {Peter C.} and Eshleman, {James R.} and Murphy, {Kathleen M.} and Berg, {Karin D.}",
year = "2003",
month = "3",
day = "1",
language = "English (US)",
volume = "83",
pages = "419--428",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Molecular diagnosis of oligodendroglioma in paraffin sections

AU - Hatanpaa, Kimmo J.

AU - Burger, Peter C.

AU - Eshleman, James R.

AU - Murphy, Kathleen M.

AU - Berg, Karin D.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Distinction of oligodendrogliomas from other gliomas is clinically important, but the histologic diagnosis of oligodendroglioma has been a difficult and notoriously subjective task. Testing for loss of heterozygosity (LOH) on chromosomal arms 1p and 19q, the genetic signature of oligodendroglioma, has emerged as a useful, objective adjunct to the traditional histologic evaluation. However, LOH testing of glioma specimens has not yet been widely implemented, presumably because of a lack of a practical LOH assay. We describe a 1p and 19q LOH assay suitable for routine diagnostics. In contrast to traditional microsatellite-based LOH analysis, we show that detection of LOH is usually possible even without normal tissue or blood from the same patient. A small area of tumor on a single paraffin section is sufficient for the assay. The assay protocol consists of a one-step DNA extraction, multiplex PCR for microsatellites on 1p and 19q, and capillary electrophoresis of the PCR products. LOH is detected by analysis of the allelic patterns and by integration of data from multiple highly polymorphic microsatellites. In a validation study on 19 gliomas, the results were concordant with results obtained by established methods and correlated well with histologic diagnoses. Because only a paraffin section is required, the pathologist can perform both the traditional histopathologic evaluation and this supporting molecular assay from the material at hand.

AB - Distinction of oligodendrogliomas from other gliomas is clinically important, but the histologic diagnosis of oligodendroglioma has been a difficult and notoriously subjective task. Testing for loss of heterozygosity (LOH) on chromosomal arms 1p and 19q, the genetic signature of oligodendroglioma, has emerged as a useful, objective adjunct to the traditional histologic evaluation. However, LOH testing of glioma specimens has not yet been widely implemented, presumably because of a lack of a practical LOH assay. We describe a 1p and 19q LOH assay suitable for routine diagnostics. In contrast to traditional microsatellite-based LOH analysis, we show that detection of LOH is usually possible even without normal tissue or blood from the same patient. A small area of tumor on a single paraffin section is sufficient for the assay. The assay protocol consists of a one-step DNA extraction, multiplex PCR for microsatellites on 1p and 19q, and capillary electrophoresis of the PCR products. LOH is detected by analysis of the allelic patterns and by integration of data from multiple highly polymorphic microsatellites. In a validation study on 19 gliomas, the results were concordant with results obtained by established methods and correlated well with histologic diagnoses. Because only a paraffin section is required, the pathologist can perform both the traditional histopathologic evaluation and this supporting molecular assay from the material at hand.

UR - http://www.scopus.com/inward/record.url?scp=0037345970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037345970&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 419

EP - 428

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 3

ER -