TY - JOUR
T1 - Molecular ordering of apoptotic mammalian CED-3/ICE-like proteases
AU - Orth, Kim
AU - O'Rourke, Karen
AU - Salvesen, Guy S.
AU - Dixit, Vishva M.
PY - 1996
Y1 - 1996
N2 - Apoptosis is executed by cysteine proteases belonging to the CED-3/ICE family, which, unlike other mammalian cysteine proteases, cleave their substrates following aspartate residues. Proteases belonging to this family exist in the cytosol as zymogens that require accurate processing at internal aspartate residues to generate the two-chain active enzymes. As such, CED- 3/ICE family members are capable of activating each other in a manner analogous to the protease zymogens of the coagulation or complement cascades. At present, it is unknown whether such mutual processing exists in vive, and if so whether it is sequential, implying an order to the death pathway. Using a cell-free apoptosis system, recombinant ICE proteases and both biochemical and morphological criteria, we demonstrate an ordering of the mammalian ICEs that are most related to the Caenorhabditis elegans death protease CED-3.
AB - Apoptosis is executed by cysteine proteases belonging to the CED-3/ICE family, which, unlike other mammalian cysteine proteases, cleave their substrates following aspartate residues. Proteases belonging to this family exist in the cytosol as zymogens that require accurate processing at internal aspartate residues to generate the two-chain active enzymes. As such, CED- 3/ICE family members are capable of activating each other in a manner analogous to the protease zymogens of the coagulation or complement cascades. At present, it is unknown whether such mutual processing exists in vive, and if so whether it is sequential, implying an order to the death pathway. Using a cell-free apoptosis system, recombinant ICE proteases and both biochemical and morphological criteria, we demonstrate an ordering of the mammalian ICEs that are most related to the Caenorhabditis elegans death protease CED-3.
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U2 - 10.1074/jbc.271.35.20977
DO - 10.1074/jbc.271.35.20977
M3 - Article
C2 - 8702858
AN - SCOPUS:0029787268
SN - 0021-9258
VL - 271
SP - 20977
EP - 20980
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -