Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases

Martin Oppermann, María Diversé-Pierluissi, Mark H. Drazner, Sara L. Dyer, Neil J. Freedman, Karsten C. Peppel, Robert J. Lefkowitz

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Abstract

Guanine nucleotide-binding regulatory protein (G protein)-coupled receptor kinases (GRKs) constitute a family of serine/threonine kinases that play a major role in the agonist-induced phosphorylation and desensitization of G-protein-coupled receptors. Herein we describe the generation of monoclonal antibodies (mAbs) that specifically react with GRK2 and GRK3 or with GRK4, GRKS, and GRK6. They are used in several different receptor systems to identify the kinases that are responsible for receptor phosphorylation and desensitization. The ability of these reagents to inhibit GRK-mediated receptor phosphorylation is demonstrated in permeabilized 293 cells that overexpress individual GRKs and the type 1A angiotensin II receptor. We also use this approach to identify the endogenous GRKs that are responsible for the agonist-induced phosphorylation of epitope-tagged β2-adrenergic receptors (β2ARs) overexpressed in rabbit ventricular myocytes that are infected with a recombinant adenovirus. In these myocytes, anti-GRK2/3 mAbs inhibit isoproterenol-induced receptor phosphorylation by 77%, while GRK4-6-specific mAbs have no effect. Consistent with the operation of a βAR kinase-mediated mechanism, GRK2 is identified by immuoblot analysis as well as in a functional assay as the predominant GRK expressed in these cells. Microinjection of GRK2/3-specific mAbs into chicken sensory neurons, which have been shown to express a GRK3-like protein, abolishes desensitization of the α2AR-mediated calcium current inhibition. The intracellular inhibition of endogenous GRKs by mAbs represents a novel approach to the study of receptor specificities among GRKs that should be widely applicable to many G-protein-coupled receptors.

Original languageEnglish (US)
Pages (from-to)7649-7654
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number15
StatePublished - Jul 23 1996

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G-Protein-Coupled Receptor Kinases
Monoclonal Antibodies
Phosphorylation
G-Protein-Coupled Receptors
Muscle Cells
Phosphotransferases
Angiotensin Type 1 Receptor
Protein-Serine-Threonine Kinases
Microinjections
Sensory Receptor Cells
Isoproterenol
GTP-Binding Proteins
Adenoviridae
Adrenergic Receptors
Epitopes
Chickens
Carrier Proteins
Rabbits
Calcium

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Oppermann, M., Diversé-Pierluissi, M., Drazner, M. H., Dyer, S. L., Freedman, N. J., Peppel, K. C., & Lefkowitz, R. J. (1996). Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7649-7654.

Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. / Oppermann, Martin; Diversé-Pierluissi, María; Drazner, Mark H.; Dyer, Sara L.; Freedman, Neil J.; Peppel, Karsten C.; Lefkowitz, Robert J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 15, 23.07.1996, p. 7649-7654.

Research output: Contribution to journalArticle

Oppermann, M, Diversé-Pierluissi, M, Drazner, MH, Dyer, SL, Freedman, NJ, Peppel, KC & Lefkowitz, RJ 1996, 'Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases', Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 15, pp. 7649-7654.
Oppermann, Martin ; Diversé-Pierluissi, María ; Drazner, Mark H. ; Dyer, Sara L. ; Freedman, Neil J. ; Peppel, Karsten C. ; Lefkowitz, Robert J. / Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 15. pp. 7649-7654.
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