Monoclonal antibodies that demonstrate specificity for several types of human lung cancer

F. Cuttitta, S. Rosen, A. F. Gazdar, J. D. Minna

Research output: Contribution to journalArticle

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Abstract

Monoclonal antibodies with selectivity for human lung cancer were produced by immunizing BALB/c mice with an established line of human small cell lung cancer (NCI-H69) and fusing the mouse spleen cells to mouse myeloma line X63-Ag8.653. The resulting hybrid cells were initially screened by immunoautoradiography for production of antibodies that would react with NCI-H69 and another small cell lung cancer line (NCI-H128) but not its autologous B-lymphoblastoid line (NCI-H128BL). Stable monoclonal antibody-producing lines were isolated by repeated cloning. Three independently derived monoclonal antibodies, designated 525A5, 534F8, and 538F12, were found to react with three of the major types of human lung cancer (small cell, adenocarcinoma, and squamous carcinoma). They did not react with bronchioloalveolar and large cell lung cancers, myeloma, lymphomas, leukemias, osteogeneic sarcoma, mesothelioma, hypernephroma, malignant melanoma, simian virus 40-transformed human fetal lung cells, skin fibroblast lines, human B-lymphoblastoid lines, human erythrocytes, and rodent cells. Interestingly, these antibodies also bound to three out of three human neuroblastomas and two out of three breast cancers but failed to react with mouse neuroblastoma and rat pheochromocytoma. The monoclonal antibodies reacted with human small cell lung cancer tumors obtained at autopsy, but had insignificant reactions with normal human lung, liver, spleen, and skeletal muscle. We conclude that monoclonal antibodies have been generated that react with common antigenic determinants expressed on several human lung cancer types, neuroblastoma, and some breast cancer, but are not detectable by our current assays on a variety of other human tumors or normal adult human tissues. Such antibodies are of potential clinical and biological importance.

Original languageEnglish (US)
Pages (from-to)4591-4595
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number7 I
StatePublished - 1981

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Antibody Specificity
Lung Neoplasms
Monoclonal Antibodies
Small Cell Lung Carcinoma
Neuroblastoma
Spleen
Breast Neoplasms
Lung
Small Cell Carcinoma
Simian virus 40
Antibodies
Hybrid Cells
Mesothelioma
Pheochromocytoma
Osteosarcoma
Renal Cell Carcinoma
Antibody Formation
Organism Cloning
Epitopes
Squamous Cell Carcinoma

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer. / Cuttitta, F.; Rosen, S.; Gazdar, A. F.; Minna, J. D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 7 I, 1981, p. 4591-4595.

Research output: Contribution to journalArticle

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