Monoclonal antibodies to human transglutaminase 4

Sung Yup Cho, Ju Hong Jeon, Chai Wan Kim, Dong Myung Shin, Gi Yong Jang, Eui Man Jeong, Sang Eun Lee, Kye Yong Song, In Gyu Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transglutaminase 4 (TG4) is a member of the enzyme family that catalyzes the calcium-dependent post-translational modification of proteins via cross-linking, polyamination, or deamidation. TG4 exhibits prostate-specific expression pattern and plays a crucial role in the formation of the copulatory plug in rodents. However, the physiological function(s) of human TG4 remains speculative. Human TG4 has been postulated to participate in the maturation process of sperm by modifying its cell surface, which results in suppression of sperm antigenicity in the female genital tract. To better understand the pathophysiological role of TG4 in prostate tissue, we generated monoclonal antibodies (MAb) against human TG4 in mice by repeated injections with the recombinant human TG4. Western blot analysis demonstrated that the selected MAbs react specifically with TG4, but not with other isoenzymes of the TG family. Immunocytochemical and immunohistochemical analyses showed that specific staining is observed with the cells overexpressing TG4 and with the paraffin-embedded prostate tissue specimens obtained from the benign prostate hyperplasia and prostate cancer patients, respectively. Our results indicate that these MAbs are suitable for detecting TG4 in the cultured cells or prostate tissues for investigating the biological functions of human TG4.

Original languageEnglish (US)
Pages (from-to)263-267
Number of pages5
JournalHybridoma
Volume29
Issue number3
DOIs
StatePublished - Jun 1 2010

Fingerprint

Monoclonal Antibodies
Prostate
transglutaminase 4
Sperm Maturation
Post Translational Protein Processing
Paraffin
Isoenzymes
Hyperplasia
Spermatozoa
Cultured Cells
Rodentia
Prostatic Neoplasms
Western Blotting
Staining and Labeling
Calcium
Injections
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Cho, S. Y., Jeon, J. H., Kim, C. W., Shin, D. M., Jang, G. Y., Jeong, E. M., ... Kim, I. G. (2010). Monoclonal antibodies to human transglutaminase 4. Hybridoma, 29(3), 263-267. https://doi.org/10.1089/hyb.2009.0112

Monoclonal antibodies to human transglutaminase 4. / Cho, Sung Yup; Jeon, Ju Hong; Kim, Chai Wan; Shin, Dong Myung; Jang, Gi Yong; Jeong, Eui Man; Lee, Sang Eun; Song, Kye Yong; Kim, In Gyu.

In: Hybridoma, Vol. 29, No. 3, 01.06.2010, p. 263-267.

Research output: Contribution to journalArticle

Cho, SY, Jeon, JH, Kim, CW, Shin, DM, Jang, GY, Jeong, EM, Lee, SE, Song, KY & Kim, IG 2010, 'Monoclonal antibodies to human transglutaminase 4', Hybridoma, vol. 29, no. 3, pp. 263-267. https://doi.org/10.1089/hyb.2009.0112
Cho SY, Jeon JH, Kim CW, Shin DM, Jang GY, Jeong EM et al. Monoclonal antibodies to human transglutaminase 4. Hybridoma. 2010 Jun 1;29(3):263-267. https://doi.org/10.1089/hyb.2009.0112
Cho, Sung Yup ; Jeon, Ju Hong ; Kim, Chai Wan ; Shin, Dong Myung ; Jang, Gi Yong ; Jeong, Eui Man ; Lee, Sang Eun ; Song, Kye Yong ; Kim, In Gyu. / Monoclonal antibodies to human transglutaminase 4. In: Hybridoma. 2010 ; Vol. 29, No. 3. pp. 263-267.
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