Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor

Herbert K. Oie, Adi F. Gazdar, Peter A. Lalley, Edward K. Russell, John D. Minna, Joseph Delarco, George J. Todaro, Uta Francke

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

INFECTION of cells with murine leukaemia virus (MuLV) strains involves an initial interaction between the major viral glycoprotein (gp71) and specific cell surface receptors1. Virus adsorption and penetration occur only in cells having specific receptors2. Other cellular factors such as virogene integration sites may also be required for productive infections. There are three major classes of MuLV: ecotropic viruses which replicate preferentially in murine cells; xenotropic viruses which do not replicate in mouse cells but can replicate in cells from other species; and amphotropic viruses which replicate in mouse cells as well as in ells from other species. None of the three classes of virus infect hamster cells3,4. Previous studies with hamster × mouse somatic cell hybrid clones segregating mouse chromosomes showed that replication of ecotropic and amphotropic viruses required a cell function(s) coded by genes assigned to mouse chromosomes 5 and 8, respectively3. However, the function(s) of these genes was not determined. The third class of MuLV, the xenotropic viruses, could not be tested in this system since both parent cells were resistant5. In the present study, the replication patterns of ecotropic and amphotropic strains were correlated with the ability of hamster x mouse hybrid clones to bind gp71 of Rauscher leukaemia virus (RLV), an ecotropic strain of MuLV. By comparing the mouse chromosome segregation patterns of hybrid clones which retained the gp71 binding ability with those clones which had lost it, we have been able to assign the gene(s) coding for RLV cell surface receptor to mouse chromosome 5.

Original languageEnglish (US)
Pages (from-to)60-62
Number of pages3
JournalNature
Volume274
Issue number5666
DOIs
StatePublished - 1978

Fingerprint

Murine Leukemia Viruses
Chromosomes, Human, Pair 5
Cell Surface Receptors
Viruses
Clone Cells
Rauscher Virus
Cricetinae
Genes
Chromosomes, Human, Pair 8
Chromosome Segregation
Hybrid Cells
Adsorption
Glycoproteins
Chromosomes

ASJC Scopus subject areas

  • General

Cite this

Oie, H. K., Gazdar, A. F., Lalley, P. A., Russell, E. K., Minna, J. D., Delarco, J., ... Francke, U. (1978). Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor. Nature, 274(5666), 60-62. https://doi.org/10.1038/274060a0

Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor. / Oie, Herbert K.; Gazdar, Adi F.; Lalley, Peter A.; Russell, Edward K.; Minna, John D.; Delarco, Joseph; Todaro, George J.; Francke, Uta.

In: Nature, Vol. 274, No. 5666, 1978, p. 60-62.

Research output: Contribution to journalArticle

Oie, HK, Gazdar, AF, Lalley, PA, Russell, EK, Minna, JD, Delarco, J, Todaro, GJ & Francke, U 1978, 'Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor', Nature, vol. 274, no. 5666, pp. 60-62. https://doi.org/10.1038/274060a0
Oie, Herbert K. ; Gazdar, Adi F. ; Lalley, Peter A. ; Russell, Edward K. ; Minna, John D. ; Delarco, Joseph ; Todaro, George J. ; Francke, Uta. / Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor. In: Nature. 1978 ; Vol. 274, No. 5666. pp. 60-62.
@article{6b6b3adcc8cf471f9d8502210eb49d9f,
title = "Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor",
abstract = "INFECTION of cells with murine leukaemia virus (MuLV) strains involves an initial interaction between the major viral glycoprotein (gp71) and specific cell surface receptors1. Virus adsorption and penetration occur only in cells having specific receptors2. Other cellular factors such as virogene integration sites may also be required for productive infections. There are three major classes of MuLV: ecotropic viruses which replicate preferentially in murine cells; xenotropic viruses which do not replicate in mouse cells but can replicate in cells from other species; and amphotropic viruses which replicate in mouse cells as well as in ells from other species. None of the three classes of virus infect hamster cells3,4. Previous studies with hamster × mouse somatic cell hybrid clones segregating mouse chromosomes showed that replication of ecotropic and amphotropic viruses required a cell function(s) coded by genes assigned to mouse chromosomes 5 and 8, respectively3. However, the function(s) of these genes was not determined. The third class of MuLV, the xenotropic viruses, could not be tested in this system since both parent cells were resistant5. In the present study, the replication patterns of ecotropic and amphotropic strains were correlated with the ability of hamster x mouse hybrid clones to bind gp71 of Rauscher leukaemia virus (RLV), an ecotropic strain of MuLV. By comparing the mouse chromosome segregation patterns of hybrid clones which retained the gp71 binding ability with those clones which had lost it, we have been able to assign the gene(s) coding for RLV cell surface receptor to mouse chromosome 5.",
author = "Oie, {Herbert K.} and Gazdar, {Adi F.} and Lalley, {Peter A.} and Russell, {Edward K.} and Minna, {John D.} and Joseph Delarco and Todaro, {George J.} and Uta Francke",
year = "1978",
doi = "10.1038/274060a0",
language = "English (US)",
volume = "274",
pages = "60--62",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5666",

}

TY - JOUR

T1 - Mouse chromosome 5 codes for ecotropic murine leukaemia virus cell-surface receptor

AU - Oie, Herbert K.

AU - Gazdar, Adi F.

AU - Lalley, Peter A.

AU - Russell, Edward K.

AU - Minna, John D.

AU - Delarco, Joseph

AU - Todaro, George J.

AU - Francke, Uta

PY - 1978

Y1 - 1978

N2 - INFECTION of cells with murine leukaemia virus (MuLV) strains involves an initial interaction between the major viral glycoprotein (gp71) and specific cell surface receptors1. Virus adsorption and penetration occur only in cells having specific receptors2. Other cellular factors such as virogene integration sites may also be required for productive infections. There are three major classes of MuLV: ecotropic viruses which replicate preferentially in murine cells; xenotropic viruses which do not replicate in mouse cells but can replicate in cells from other species; and amphotropic viruses which replicate in mouse cells as well as in ells from other species. None of the three classes of virus infect hamster cells3,4. Previous studies with hamster × mouse somatic cell hybrid clones segregating mouse chromosomes showed that replication of ecotropic and amphotropic viruses required a cell function(s) coded by genes assigned to mouse chromosomes 5 and 8, respectively3. However, the function(s) of these genes was not determined. The third class of MuLV, the xenotropic viruses, could not be tested in this system since both parent cells were resistant5. In the present study, the replication patterns of ecotropic and amphotropic strains were correlated with the ability of hamster x mouse hybrid clones to bind gp71 of Rauscher leukaemia virus (RLV), an ecotropic strain of MuLV. By comparing the mouse chromosome segregation patterns of hybrid clones which retained the gp71 binding ability with those clones which had lost it, we have been able to assign the gene(s) coding for RLV cell surface receptor to mouse chromosome 5.

AB - INFECTION of cells with murine leukaemia virus (MuLV) strains involves an initial interaction between the major viral glycoprotein (gp71) and specific cell surface receptors1. Virus adsorption and penetration occur only in cells having specific receptors2. Other cellular factors such as virogene integration sites may also be required for productive infections. There are three major classes of MuLV: ecotropic viruses which replicate preferentially in murine cells; xenotropic viruses which do not replicate in mouse cells but can replicate in cells from other species; and amphotropic viruses which replicate in mouse cells as well as in ells from other species. None of the three classes of virus infect hamster cells3,4. Previous studies with hamster × mouse somatic cell hybrid clones segregating mouse chromosomes showed that replication of ecotropic and amphotropic viruses required a cell function(s) coded by genes assigned to mouse chromosomes 5 and 8, respectively3. However, the function(s) of these genes was not determined. The third class of MuLV, the xenotropic viruses, could not be tested in this system since both parent cells were resistant5. In the present study, the replication patterns of ecotropic and amphotropic strains were correlated with the ability of hamster x mouse hybrid clones to bind gp71 of Rauscher leukaemia virus (RLV), an ecotropic strain of MuLV. By comparing the mouse chromosome segregation patterns of hybrid clones which retained the gp71 binding ability with those clones which had lost it, we have been able to assign the gene(s) coding for RLV cell surface receptor to mouse chromosome 5.

UR - http://www.scopus.com/inward/record.url?scp=0018102954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018102954&partnerID=8YFLogxK

U2 - 10.1038/274060a0

DO - 10.1038/274060a0

M3 - Article

C2 - 566388

AN - SCOPUS:0018102954

VL - 274

SP - 60

EP - 62

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5666

ER -