Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains

Mark H. Siegelman, Matthijs Van De Rijn, Irving L. Weissman

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

Isolation of a clone encoding the mouse lymph node homing receptor reveals a deduced protein with an unusual protein mosaic architecture, containing a separate carbohydrate-binding (lectin) domain, an epidermal growth factor-like (EGF) domain, and an extracellular precisely duplicated repeat unit, which preserves the motif seen in the homologous repeat structure of complement regulatory proteins and other proteins. The receptor molecule is potentially highly glycosylated, and contains an apparent transmembrane region. Analysis of messenger RNA transcripts reveals a predominantly lymphoid distribution in direct relation to the cell surface expression of the MEL-14 determinant, and the cDNA done is shown to confer the MEL-14 epitope in heterologous cells. The many novel features, induding ubiquitination, embodied in this single receptor molecule form the basis for numerous approaches to the study of cell-cell interactions.

Original languageEnglish (US)
Pages (from-to)1165-1172
Number of pages8
JournalScience
Volume243
Issue number4895
StatePublished - 1989

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Lymphocyte Homing Receptors
Glycoproteins
Complementary DNA
Clone Cells
Proteins
Ubiquitination
Epidermal Growth Factor
Lectins
Cell Communication
Epitopes
Complement System Proteins
Carbohydrates
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Siegelman, M. H., Van De Rijn, M., & Weissman, I. L. (1989). Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains. Science, 243(4895), 1165-1172.

Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains. / Siegelman, Mark H.; Van De Rijn, Matthijs; Weissman, Irving L.

In: Science, Vol. 243, No. 4895, 1989, p. 1165-1172.

Research output: Contribution to journalArticle

Siegelman, MH, Van De Rijn, M & Weissman, IL 1989, 'Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains', Science, vol. 243, no. 4895, pp. 1165-1172.
Siegelman, Mark H. ; Van De Rijn, Matthijs ; Weissman, Irving L. / Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains. In: Science. 1989 ; Vol. 243, No. 4895. pp. 1165-1172.
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