Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains

Mark H. Siegelman, Matthijs Van De Rijn, Irving L. Weissman

Research output: Contribution to journalArticle

302 Scopus citations

Abstract

Isolation of a clone encoding the mouse lymph node homing receptor reveals a deduced protein with an unusual protein mosaic architecture, containing a separate carbohydrate-binding (lectin) domain, an epidermal growth factor-like (EGF) domain, and an extracellular precisely duplicated repeat unit, which preserves the motif seen in the homologous repeat structure of complement regulatory proteins and other proteins. The receptor molecule is potentially highly glycosylated, and contains an apparent transmembrane region. Analysis of messenger RNA transcripts reveals a predominantly lymphoid distribution in direct relation to the cell surface expression of the MEL-14 determinant, and the cDNA done is shown to confer the MEL-14 epitope in heterologous cells. The many novel features, induding ubiquitination, embodied in this single receptor molecule form the basis for numerous approaches to the study of cell-cell interactions.

Original languageEnglish (US)
Pages (from-to)1165-1172
Number of pages8
JournalScience
Volume243
Issue number4895
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • General

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