mRNA expression of renal urea transporters in normal and brattleboro rats

Effect of dietary protein intake

Ming C Hu, Lise Bankir, Marie Marcelle Trinh-Trang-Tan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Differences in dietary protein level induce differences in fractional excretion of urea, in arginine vasopressin (AVP) plasma level, and in urine concentrating activity (in which intervene the renal urea transporters (UT)). The abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMCD) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high protein diet. The implication of AVP was then examined by studying AVP- deprived (Brattleboro) rats. Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in terminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). These data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) AVP influences the mRNA abundance of the UT-A1 of the terminal IMCD during protein deficiency.

Original languageEnglish (US)
Pages (from-to)44-51
Number of pages8
JournalExperimental Nephrology
Volume7
Issue number1
StatePublished - Jan 1999

Fingerprint

Brattleboro Rats
Dietary Proteins
Arginine Vasopressin
Kidney
Messenger RNA
Protein Deficiency
varespladib methyl
Kidney Medulla
Protein-Restricted Diet
urea transporter
Rectum
Sprague Dawley Rats
Urea
Proteins
Extremities
Urine
RNA
Diet

Keywords

  • Arginine vasopressin
  • Brattleboro rats
  • Dietary protein
  • Kidney
  • Messenger RNA
  • Urea excretion
  • Urea transporters
  • Urine concentrating activity

ASJC Scopus subject areas

  • Nephrology

Cite this

mRNA expression of renal urea transporters in normal and brattleboro rats : Effect of dietary protein intake. / Hu, Ming C; Bankir, Lise; Trinh-Trang-Tan, Marie Marcelle.

In: Experimental Nephrology, Vol. 7, No. 1, 01.1999, p. 44-51.

Research output: Contribution to journalArticle

@article{279c578578004c27866a7c792395f8bd,
title = "mRNA expression of renal urea transporters in normal and brattleboro rats: Effect of dietary protein intake",
abstract = "Differences in dietary protein level induce differences in fractional excretion of urea, in arginine vasopressin (AVP) plasma level, and in urine concentrating activity (in which intervene the renal urea transporters (UT)). The abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMCD) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high protein diet. The implication of AVP was then examined by studying AVP- deprived (Brattleboro) rats. Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in terminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). These data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) AVP influences the mRNA abundance of the UT-A1 of the terminal IMCD during protein deficiency.",
keywords = "Arginine vasopressin, Brattleboro rats, Dietary protein, Kidney, Messenger RNA, Urea excretion, Urea transporters, Urine concentrating activity",
author = "Hu, {Ming C} and Lise Bankir and Trinh-Trang-Tan, {Marie Marcelle}",
year = "1999",
month = "1",
language = "English (US)",
volume = "7",
pages = "44--51",
journal = "Experimental Nephrology",
issn = "0028-2766",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - mRNA expression of renal urea transporters in normal and brattleboro rats

T2 - Effect of dietary protein intake

AU - Hu, Ming C

AU - Bankir, Lise

AU - Trinh-Trang-Tan, Marie Marcelle

PY - 1999/1

Y1 - 1999/1

N2 - Differences in dietary protein level induce differences in fractional excretion of urea, in arginine vasopressin (AVP) plasma level, and in urine concentrating activity (in which intervene the renal urea transporters (UT)). The abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMCD) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high protein diet. The implication of AVP was then examined by studying AVP- deprived (Brattleboro) rats. Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in terminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). These data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) AVP influences the mRNA abundance of the UT-A1 of the terminal IMCD during protein deficiency.

AB - Differences in dietary protein level induce differences in fractional excretion of urea, in arginine vasopressin (AVP) plasma level, and in urine concentrating activity (in which intervene the renal urea transporters (UT)). The abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMCD) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high protein diet. The implication of AVP was then examined by studying AVP- deprived (Brattleboro) rats. Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in terminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). These data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) AVP influences the mRNA abundance of the UT-A1 of the terminal IMCD during protein deficiency.

KW - Arginine vasopressin

KW - Brattleboro rats

KW - Dietary protein

KW - Kidney

KW - Messenger RNA

KW - Urea excretion

KW - Urea transporters

KW - Urine concentrating activity

UR - http://www.scopus.com/inward/record.url?scp=0345222489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345222489&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 44

EP - 51

JO - Experimental Nephrology

JF - Experimental Nephrology

SN - 0028-2766

IS - 1

ER -