mRNA expression of renal urea transporters in normal and brattleboro rats: Effect of dietary protein intake

Ming C Hu, Lise Bankir, Marie Marcelle Trinh-Trang-Tan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Differences in dietary protein level induce differences in fractional excretion of urea, in arginine vasopressin (AVP) plasma level, and in urine concentrating activity (in which intervene the renal urea transporters (UT)). The abundance of mRNA for UT-A1 (of the inner medullary collecting duct, IMCD) UT-A2 (of the descending thin limb) and UT-B1 (of descending vasa recta) was determined by Northern analysis of total RNA extracted from medullary subregions of Sprague-Dawley rats fed for 1 week, a low, normal, or high protein diet. The implication of AVP was then examined by studying AVP- deprived (Brattleboro) rats. Our results show that none of these transporters is affected by the level of protein intake, except UT-A1 that is reduced in terminal IMCD by low protein diet in the absence of AVP (Brattleboro rats). These data suggest that (1) the previously reported effect of kidney medulla hypertonicity on UT-A2 and UT-B1 mRNA expression is somehow obliterated by protein intake deficiency or excess, and (2) AVP influences the mRNA abundance of the UT-A1 of the terminal IMCD during protein deficiency.

Original languageEnglish (US)
Pages (from-to)44-51
Number of pages8
JournalExperimental Nephrology
Volume7
Issue number1
StatePublished - Jan 1 1999

Keywords

  • Arginine vasopressin
  • Brattleboro rats
  • Dietary protein
  • Kidney
  • Messenger RNA
  • Urea excretion
  • Urea transporters
  • Urine concentrating activity

ASJC Scopus subject areas

  • Nephrology

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