Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis

Laura L. Carter, Thomas E. Redelmeier, Leslie A. Woollenweber, Sandra L. Schmid

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTP-γS, AlF4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, AlF4 -, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalJournal of Cell Biology
Volume120
Issue number1
StatePublished - Jan 1993

Fingerprint

Clathrin-Coated Vesicles
Endocytosis
GTP-Binding Proteins
Coated Vesicles
Guanosine Triphosphate
Heterotrimeric GTP-Binding Proteins
Clathrin
tetrafluoroaluminate
mastoparan

ASJC Scopus subject areas

  • Cell Biology

Cite this

Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis. / Carter, Laura L.; Redelmeier, Thomas E.; Woollenweber, Leslie A.; Schmid, Sandra L.

In: Journal of Cell Biology, Vol. 120, No. 1, 01.1993, p. 37-45.

Research output: Contribution to journalArticle

Carter, LL, Redelmeier, TE, Woollenweber, LA & Schmid, SL 1993, 'Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis', Journal of Cell Biology, vol. 120, no. 1, pp. 37-45.
Carter, Laura L. ; Redelmeier, Thomas E. ; Woollenweber, Leslie A. ; Schmid, Sandra L. / Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis. In: Journal of Cell Biology. 1993 ; Vol. 120, No. 1. pp. 37-45.
@article{3fb529c797354de0bd8532176be0fc7d,
title = "Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis",
abstract = "We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTP-γS, AlF4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, AlF4 -, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.",
author = "Carter, {Laura L.} and Redelmeier, {Thomas E.} and Woollenweber, {Leslie A.} and Schmid, {Sandra L.}",
year = "1993",
month = "1",
language = "English (US)",
volume = "120",
pages = "37--45",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis

AU - Carter, Laura L.

AU - Redelmeier, Thomas E.

AU - Woollenweber, Leslie A.

AU - Schmid, Sandra L.

PY - 1993/1

Y1 - 1993/1

N2 - We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTP-γS, AlF4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, AlF4 -, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.

AB - We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTP-γS, AlF4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, AlF4 -, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.

UR - http://www.scopus.com/inward/record.url?scp=0027498027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027498027&partnerID=8YFLogxK

M3 - Article

VL - 120

SP - 37

EP - 45

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -