Multiple mRNAs for 3-hydroxy-3-methylglutaryl coenzyme A reductase determined by multiple transcription initiation sites and intron splicing sites in the 5'-untranslated region

G. A. Reynolds, J. L. Goldstein, M. S. Brown

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The current studies show that mRNAs with 16 different 5'-untranslated regions (varying in length from 68 to 670 nucleotides) are produced from the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in hamster UT-1 cells. This complex pattern of mRNAs results from a combination of multiple transcription initiation sites and multiple 5' splice donor sites for the intron in the 5'-untranslated region of the gene. Analysis of the multiple mRNAs was made possible by a modification of the S1 nuclease technique in which we used a series of progressively truncated uniformly labeled, single-stranded [32P]DNA probes in addition to the usual end-labeled 32P-probes. All of the reductase mRNAs are diminished when UT-1 cells are incubated with sterols, indicating that all of them are subject to coordinate control.

Original languageEnglish (US)
Pages (from-to)10369-10377
Number of pages9
JournalJournal of Biological Chemistry
Volume260
Issue number18
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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