Abstract
The current studies show that mRNAs with 16 different 5'-untranslated regions (varying in length from 68 to 670 nucleotides) are produced from the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in hamster UT-1 cells. This complex pattern of mRNAs results from a combination of multiple transcription initiation sites and multiple 5' splice donor sites for the intron in the 5'-untranslated region of the gene. Analysis of the multiple mRNAs was made possible by a modification of the S1 nuclease technique in which we used a series of progressively truncated uniformly labeled, single-stranded [32P]DNA probes in addition to the usual end-labeled 32P-probes. All of the reductase mRNAs are diminished when UT-1 cells are incubated with sterols, indicating that all of them are subject to coordinate control.
Original language | English (US) |
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Pages (from-to) | 10369-10377 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 260 |
Issue number | 18 |
State | Published - 1985 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology