Multiple ras functions can contribute to mammalian cell transformation

Michael A. White, Charles Nicolette, Audrey Minden, Anthony Polverino, Linda Van Aelst, Michael Karin, Michael H. Wigler

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606 Scopus citations

Abstract

We have developed a generalized approach, using two hybrid interactions, to isolate Ha-Ras effector loop mutations that separate the ability of Ha-Ras to interact with different downstream effectors. These mutations attenuate or eliminate Ha-ras(G12V) transformation of mammalian cells, but retain complementary activity, as demonstrated by synergistic induction of foci of growth-transformed cells, and by the ability to activate different downstream components. The transformation defect of Ha-ras(G12V, E37G) is rescued by a mutant, raf1, that restores interaction. These results indicate that multiple cellular components, including Raf1, are activated by Ha-Ras and contribute to Ha-Ras-induced mammalian cell transformation.

Original languageEnglish (US)
Pages (from-to)533-541
Number of pages9
JournalCell
Volume80
Issue number4
DOIs
StatePublished - Feb 24 1995

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

White, M. A., Nicolette, C., Minden, A., Polverino, A., Aelst, L. V., Karin, M., & Wigler, M. H. (1995). Multiple ras functions can contribute to mammalian cell transformation. Cell, 80(4), 533-541. https://doi.org/10.1016/0092-8674(95)90507-3