Multiple roles for acetylation in the interaction of p300 HAT with ATF-2

Balasubramanyam Karanam, Ling Wang, Dongxia Wang, Xin Liu, Ronen Marmorstein, Robert Cotter, Philip A. Cole

Research output: Contribution to journalArticle

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Abstract

The transcriptional coactivator paralogues p300 and CBP contain acetyltransferase domains (HAT) and catalyze the lysine acetylation of histones and other proteins as an important aspect of their functions. Prior studies revealed that the basic leucine zipper domain (b-ZIP) of transcription factor ATF-2 (also called CRE-BP1) can interact with the CBP HAT domain. In this study, we have examined the ATF-2 b-ZIP interaction with the p300 HAT domain and shown that p300 HAT autoacetylation can enhance the binding affinity. Pull-down assays revealed that hyperacetylated p300 HAT is more efficiently retained by immobilized ATF-2 b-ZIP than hypoacetylated p300 HAT. Loop deleted p300 HAT lacking autoacetylation was retained about as well as hyperacetylated p300 HAT, suggesting that the loop and ATF-2 compete for p300 HAT binding. While ATF-2 b-ZIP is a weak inhibitor of hypoacetylated p300 HAT acetylation of a histone H4 peptide, hyperacetylated p300 HAT is much more potently inhibited by ATF-2 b-ZIP. Moreover, we showed that ATF-2 b-ZIP could serve as an acetyltransferase substrate for p300 HAT. Using mass spectrometry, two p300 HAT lysine acetylation sites were mapped in ATF-2 b-ZIP. Immunoprecipitation-Western blot analysis with anti-acetyl-lysine antibody revealed that ATF-2 can undergo reversible acetylation in vivo. Mutational analysis of the two ATF-2 b-ZIP acetylation sites revealed their potential contributions to ATF-2-mediated transcriptional activation. Taken together, these studies suggest multiple roles for protein acetylation in the regulation of transcription by p300/CBP and ATF-2.

Original languageEnglish (US)
Pages (from-to)8207-8216
Number of pages10
JournalBiochemistry
Volume46
Issue number28
DOIs
StatePublished - Jul 17 2007

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Acetylation
Lysine
Acetyltransferases
Histones
p300-CBP Transcription Factors
Activating Transcription Factors
Leucine Zippers
Transcription
Immunoprecipitation
Transcriptional Activation
Mass spectrometry
Assays
Mass Spectrometry
Proteins
Western Blotting
Chemical activation
Peptides
Antibodies
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Karanam, B., Wang, L., Wang, D., Liu, X., Marmorstein, R., Cotter, R., & Cole, P. A. (2007). Multiple roles for acetylation in the interaction of p300 HAT with ATF-2. Biochemistry, 46(28), 8207-8216. https://doi.org/10.1021/bi7000054

Multiple roles for acetylation in the interaction of p300 HAT with ATF-2. / Karanam, Balasubramanyam; Wang, Ling; Wang, Dongxia; Liu, Xin; Marmorstein, Ronen; Cotter, Robert; Cole, Philip A.

In: Biochemistry, Vol. 46, No. 28, 17.07.2007, p. 8207-8216.

Research output: Contribution to journalArticle

Karanam, B, Wang, L, Wang, D, Liu, X, Marmorstein, R, Cotter, R & Cole, PA 2007, 'Multiple roles for acetylation in the interaction of p300 HAT with ATF-2', Biochemistry, vol. 46, no. 28, pp. 8207-8216. https://doi.org/10.1021/bi7000054
Karanam B, Wang L, Wang D, Liu X, Marmorstein R, Cotter R et al. Multiple roles for acetylation in the interaction of p300 HAT with ATF-2. Biochemistry. 2007 Jul 17;46(28):8207-8216. https://doi.org/10.1021/bi7000054
Karanam, Balasubramanyam ; Wang, Ling ; Wang, Dongxia ; Liu, Xin ; Marmorstein, Ronen ; Cotter, Robert ; Cole, Philip A. / Multiple roles for acetylation in the interaction of p300 HAT with ATF-2. In: Biochemistry. 2007 ; Vol. 46, No. 28. pp. 8207-8216.
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