Multiple splice forms of ribonuclease-inhibitor mRNA differ in the 5′-untranslated region

David Crawford, Kathleen Hagerty, Bruce Beutler

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We report the sequence of the cDNAs representing five independent splice forms of human placental RNase inhibitor (RI) mRNA. RI mRNAs differ principally in the 5′-untranslated region, which may include or lack a 68-nucleotide (nt) exon inserted at a splice site located only 20 nt upstream from the initiator AUG. At least three other exons may also abut the same splice site. This unusual and variable feature of the mRNA would suggest that secondary structure in the region of the start codon may differ among RI messages. A single copy of the RI gene exists in the human genome.

Original languageEnglish (US)
Pages (from-to)525-531
Number of pages7
JournalGene
Volume85
Issue number2
DOIs
StatePublished - Dec 28 1989

Keywords

  • RNase
  • Recombinant DNA
  • alterative splicing
  • cDNA
  • exon
  • human RNase inhibitor
  • start codon

ASJC Scopus subject areas

  • Genetics

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