Multiple thymine dimer excising nuclease activities in extracts of human KB cells

Kem H. Cook, Errol C. Friedberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Crude extracts of human KB cells grown in suspension culture contain enzyme activity that catalyzes the preferential excision of thymine-containing pyrimidine dimers from UV-irradiated E. coli DNA specifically incised adjacent to dimer sites. Fractionation of KB cell crude extracts reveals the presence of three such activities with distinct affinities for both DEAE-cellulose and phosphocellulose. One of the activities (activity B) is distinguished by its s20,w (2.6) and isoelectric point (9.0) from the other two (activities A and C) which have similar s20,w's (3.0-3.2) and isoelectric points (6.0). All three differ in their extent of stimulation by divalent cation and inhibition by NaCl or a sulfhydryl group inhibitor. These results indicate that multiple 5′ → 3′ dimer excision nuclease activities exist in human cells; however, there is as yet no direct evidence that these enzymes are functional in nucleotide excision repair in vivo.

Original languageEnglish (US)
Pages (from-to)850-857
Number of pages8
JournalBiochemistry
Volume17
Issue number5
StatePublished - 1978

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Pyrimidine Dimers
KB Cells
Isoelectric Point
Complex Mixtures
Dimers
Cells
DEAE-Cellulose
Thymine
Divalent Cations
Enzyme activity
Enzymes
Fractionation
Cell Extracts
Cell culture
DNA Repair
Escherichia coli
Suspensions
Repair
Nucleotides
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Multiple thymine dimer excising nuclease activities in extracts of human KB cells. / Cook, Kem H.; Friedberg, Errol C.

In: Biochemistry, Vol. 17, No. 5, 1978, p. 850-857.

Research output: Contribution to journalArticle

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