Murine macrophage autophagy protects against alcohol-induced liver injury by degrading interferon regulatory factor 1 (IRF1) and removing damaged mitochondria

Shuang Liang, Zhenyu Zhong, So Yeon Kim, Ryosuke Uchiyama, Yoon Seok Roh, Hiroshi Matsushita, Roberta A. Gottlieb, Ekihiro Seki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Excessive alcohol consumption induces intestinal dysbiosis of the gut microbiome and reduces gut epithelial integrity. This often leads to portal circulation–mediated translocation of gut-derived microbial products, such as lipopolysaccharide (LPS), to the liver, where these products engage Toll-like receptor 4 (TLR4) and initiate hepatic inflammation, which promotes alcoholic liver disease (ALD). Although the key self-destructive process of autophagy has been well-studied in hepatocytes, its role in macrophages during ALD pathogenesis remains elusive. Using WT and myeloid cell–specific autophagy-related 7 (Atg7) knockout (Atg7Mye) mice, we found that chronic ethanol feeding for 6 weeks plus LPS injection enhances serum alanine aminotransferase and IL-1 levels and augments hepatic C-C motif chemokine ligand 5 (CCL5) and C-X-C motif chemokine ligand 10 (CXCL10) expression in WT mice, a phenotype that was further exacerbated in Atg7Mye mice. Atg7Mye macrophages exhibited defective mitochondrial respiration and displayed elevated mitochondrial reactive oxygen species production and inflammasome activation relative to WT cells. Interestingly, compared with WT cells, Atg7Mye macrophages also had a drastically increased abundance and nuclear translocation of interferon regulatory factor 1 (IRF1) after LPS stimulation. Mechanistically, LPS induced co-localization of IRF1 with the autophagy adaptor p62 and the autophagosome, resulting in subsequent IRF1 degradation. However, upon p62 silencing or Atg7 deletion, IRF1 started to accumulate in autophagy-deficient macrophages and translocated into the nucleus, where it induced CCL5 and CXCL10 expression. In conclusion, macrophage autophagy protects against ALD by promoting IRF1 degradation and removal of damaged mitochondria, limiting macrophage activation and inflammation.

Original languageEnglish (US)
Pages (from-to)12359-12369
Number of pages11
JournalJournal of Biological Chemistry
Volume294
Issue number33
DOIs
StatePublished - Jan 1 2019

Fingerprint

Interferon Regulatory Factor-1
Mitochondria
Macrophages
Autophagy
Liver
Alcohols
Alcoholic Liver Diseases
Lipopolysaccharides
Wounds and Injuries
Chemokine CXCL10
Chemokines
C Chemokines
Chemical activation
Dysbiosis
Inflammasomes
Ligands
Inflammation
Degradation
CC Chemokines
Toll-Like Receptor 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Murine macrophage autophagy protects against alcohol-induced liver injury by degrading interferon regulatory factor 1 (IRF1) and removing damaged mitochondria. / Liang, Shuang; Zhong, Zhenyu; Kim, So Yeon; Uchiyama, Ryosuke; Roh, Yoon Seok; Matsushita, Hiroshi; Gottlieb, Roberta A.; Seki, Ekihiro.

In: Journal of Biological Chemistry, Vol. 294, No. 33, 01.01.2019, p. 12359-12369.

Research output: Contribution to journalArticle

Liang, Shuang ; Zhong, Zhenyu ; Kim, So Yeon ; Uchiyama, Ryosuke ; Roh, Yoon Seok ; Matsushita, Hiroshi ; Gottlieb, Roberta A. ; Seki, Ekihiro. / Murine macrophage autophagy protects against alcohol-induced liver injury by degrading interferon regulatory factor 1 (IRF1) and removing damaged mitochondria. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 33. pp. 12359-12369.
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AU - Uchiyama, Ryosuke

AU - Roh, Yoon Seok

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AU - Gottlieb, Roberta A.

AU - Seki, Ekihiro

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