Murine trophoblast can be killed by allospecific cytotoxic T lymphocytes generated in GIBCO Opti-MEM medium

Belinda L. Drake, Judith R. Head

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Previous work in this laboratory demonstrated that a population of cultured midterm murine trophoblast cells are not susceptible to lysis by allospecific cytotoxic T lymphocytes (CTL) generated by standard in vitro protocols. We now report that this trophoblast population is killed, in an MHC-dependent manner, by allospecific CTL generated in GIBCO Opti-MEM, a modified tissue culture medium designed to maintain cell growth and proliferation in the presence of low concentrations of fetal bovine serum (FBS).

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalJournal of Reproductive Immunology
Volume15
Issue number1
DOIs
StatePublished - Mar 1989

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Keywords

  • cytotoxic T lymphocytes
  • in vitro susceptibility
  • murine trophoblast

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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