Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

Martha Hotz Vitaterna; David P. King; Anne Marie Chang; Jon M. Kernhauser; Phillip L. Lowrey; J. David McDonald; William F. Dove; Lawrence H. Pinto; Fred W. Turek; Joseph S. Takahashi

doi:10.1126/science.8171325

Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

Martha Hotz Vitaterna, David P. King, Anne Marie Chang, Jon M. Kernhauser, Phillip L. Lowrey, J. David McDonald, William F. Dove, Lawrence H. Pinto, Fred W. Turek, Joseph S. Takahashi

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Abstract

In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.

Original language	English (US)
Pages (from-to)	719-725
Number of pages	7
Journal	Science
Volume	264
Issue number	5159
DOIs	https://doi.org/10.1126/science.8171325
State	Published - Apr 29 1994

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title = "Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior",

abstract = "In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.",

author = "Vitaterna, {Martha Hotz} and King, {David P.} and Chang, {Anne Marie} and Kernhauser, {Jon M.} and Lowrey, {Phillip L.} and McDonald, {J. David} and Dove, {William F.} and Pinto, {Lawrence H.} and Turek, {Fred W.} and Takahashi, {Joseph S.}",

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T1 - Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

AU - Vitaterna, Martha Hotz

AU - King, David P.

AU - Chang, Anne Marie

AU - Kernhauser, Jon M.

AU - Lowrey, Phillip L.

AU - McDonald, J. David

AU - Dove, William F.

AU - Pinto, Lawrence H.

AU - Turek, Fred W.

AU - Takahashi, Joseph S.

PY - 1994/4/29

Y1 - 1994/4/29

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AB - In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.

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Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

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