Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

Martha Hotz Vitaterna, David P. King, Anne Marie Chang, Jon M. Kernhauser, Phillip L. Lowrey, J. David McDonald, William F. Dove, Lawrence H. Pinto, Fred W. Turek, Joseph S. Takahashi

Research output: Contribution to journalArticle

1195 Scopus citations

Abstract

In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.

Original languageEnglish (US)
Pages (from-to)719-725
Number of pages7
JournalScience
Volume264
Issue number5159
DOIs
StatePublished - Apr 29 1994

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    Vitaterna, M. H., King, D. P., Chang, A. M., Kernhauser, J. M., Lowrey, P. L., McDonald, J. D., Dove, W. F., Pinto, L. H., Turek, F. W., & Takahashi, J. S. (1994). Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior. Science, 264(5159), 719-725. https://doi.org/10.1126/science.8171325