Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior

Martha Hotz Vitaterna, David P. King, Anne Marie Chang, Jon M. Kernhauser, Phillip L. Lowrey, J. David McDonald, William F. Dove, Lawrence H. Pinto, Fred W. Turek, Joseph S. Takahashi

Research output: Contribution to journalArticle

1154 Citations (Scopus)

Abstract

In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.

Original languageEnglish (US)
Pages (from-to)719-725
Number of pages7
JournalScience
Volume264
Issue number5159
StatePublished - Apr 29 1994

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Ethylnitrosourea
Circadian Clocks
Essential Genes
Mutagenesis
Mammals
Chromosomes, Human, Pair 5
Mutation
Chromosomes, Human, Pair 4
Aptitude
Gene Order
Human Chromosomes
Periodicity
Circadian Rhythm
Genes
Clone Cells
Power (Psychology)

ASJC Scopus subject areas

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Cite this

Vitaterna, M. H., King, D. P., Chang, A. M., Kernhauser, J. M., Lowrey, P. L., McDonald, J. D., ... Takahashi, J. S. (1994). Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior. Science, 264(5159), 719-725.

Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior. / Vitaterna, Martha Hotz; King, David P.; Chang, Anne Marie; Kernhauser, Jon M.; Lowrey, Phillip L.; McDonald, J. David; Dove, William F.; Pinto, Lawrence H.; Turek, Fred W.; Takahashi, Joseph S.

In: Science, Vol. 264, No. 5159, 29.04.1994, p. 719-725.

Research output: Contribution to journalArticle

Vitaterna, MH, King, DP, Chang, AM, Kernhauser, JM, Lowrey, PL, McDonald, JD, Dove, WF, Pinto, LH, Turek, FW & Takahashi, JS 1994, 'Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior', Science, vol. 264, no. 5159, pp. 719-725.
Vitaterna MH, King DP, Chang AM, Kernhauser JM, Lowrey PL, McDonald JD et al. Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior. Science. 1994 Apr 29;264(5159):719-725.
Vitaterna, Martha Hotz ; King, David P. ; Chang, Anne Marie ; Kernhauser, Jon M. ; Lowrey, Phillip L. ; McDonald, J. David ; Dove, William F. ; Pinto, Lawrence H. ; Turek, Fred W. ; Takahashi, Joseph S. / Mutagenesis and mapping of a mouse gene, clock, essential for circadian behavior. In: Science. 1994 ; Vol. 264, No. 5159. pp. 719-725.
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