Abstract
Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6-8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially designed retroviral vector that permits rapid identification of disrupted genes in each cell that bears a phenotype of interest. The utility of combined chemical and insertional mutagenesis is illustrated by the identification of novel host genes that are required for macrophage sensitivity to anthrax lethal factor.
Original language | English (US) |
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Pages (from-to) | 493-500 |
Number of pages | 8 |
Journal | BioTechniques |
Volume | 42 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology