Mutation analysis of the PTEN/MMAC1 gene in lung cancer

Eva Forgacs, Eric J. Biesterveld, Yoshitaka Sekido, Kwun Fong, Sabeeha Muneer, Ignacio I. Wistuba, Sara Milchgrub, Ruth Brezinschek, Arvind Virmani, Adi F. Gazdar, John D. Minna

Research output: Contribution to journalArticle

179 Scopus citations

Abstract

We studied PTEN/MMAC1, a newly discovered candidate tumor suppressor gene at 10q23.3, for mutations in lung cancer. One hundred and thirty-six lung cancer cell line DNAs (66 small cell lung cancers, SCLC, 61 non-small cell lung cancers, NSCLC, four mesotheliomas, five extrapulmonary small cell cancers) were analysed for PTEN/MMAC1 homozygous deletions and five (8%) SCLC lines showed homozygous deletions interrupting the PTEN/MMAC1 gene. Using single stranded conformation polymorphism (SSCP) analysis, we screened the PTEN/MMAC1 open reading frame of 53 lung cancer cell line cDNAs for point mutations and found that 3/35 SCLCs and 3/18 NSCLCs contained homozygous amino acid sequence altering mutations. Northern blot analysis revealed that expression of the PTEN/MMAC1 gene was considerably lower in all the tumor cell lines with point mutations while no expression was detected for cell lines with PTEN/MMAC1 homozygous deletions. Mutation analysis of 22 uncultured, microdissected, primary SCLC tumors and metastases showed two silent mutations, and two apparent homozygous deletions. We also discovered a processed pseudogene (PTEN2) which has 98.5% nt identity to PTEN/MMAC1, that needs to be accounted for in cDNA mutation analysis. Our findings suggest that genetic abnormalities of the PTEN/MMAC1 gene are only involved in a relatively small subset of lung cancers.

Original languageEnglish (US)
Pages (from-to)1557-1565
Number of pages9
JournalOncogene
Volume17
Issue number12
DOIs
StatePublished - Sep 24 1998

Keywords

  • Chromosome 10q23
  • Homozygous deletion
  • Loss of heterozygosity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Forgacs, E., Biesterveld, E. J., Sekido, Y., Fong, K., Muneer, S., Wistuba, I. I., Milchgrub, S., Brezinschek, R., Virmani, A., Gazdar, A. F., & Minna, J. D. (1998). Mutation analysis of the PTEN/MMAC1 gene in lung cancer. Oncogene, 17(12), 1557-1565. https://doi.org/10.1038/sj.onc.1202070