MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer

Niranjan Venkateswaran, M. Carmen Lafita-Navarro, Yi Heng Hao, Jessica A. Kilgore, Lizbeth Perez-Castro, Jonathan Braverman, Nofit Borenstein-Auerbach, Min Kim, Nicholas P. Lesner, Prashant Mishra, Thomas Brabletz, Jerry W. Shay, Ralph J. DeBerardinis, Noelle S. Williams, Omer H. Yilmaz, Maralice Conacci-Sorrell

Research output: Contribution to journalArticle

Abstract

Tumors display increased uptake and processing of nutrients to fulfill the demands of rapidly proliferating cancer cells. Seminal studies have shown that the proto-oncogene MYC promotes metabolic reprogramming by altering glutamine uptake and metabolism in cancer cells. How MYC regulates the metabolism of other amino acids in cancer is not fully understood. Using high-performance liquid chromatography (HPLC)-tandem mass spectrometry (LC-MS/MS), we found that MYC increased intracellular levels of tryptophan and tryptophan metabolites in the kynurenine pathway. MYC induced the expression of the tryptophan transporters SLC7A5 and SLC1A5 and the enzyme arylformamidase (AFMID), involved in the conversion of tryptophan into kynurenine. SLC7A5, SLC1A5, and AFMID were elevated in colon cancer cells and tissues, and kynurenine was significantly greater in tumor samples than in the respective adjacent normal tissue from patients with colon cancer. Compared with normal human colonic epithelial cells, colon cancer cells were more sensitive to the depletion of tryptophan. Blocking enzymes in the kynurenine pathway caused preferential death of established colon cancer cells and transformed colonic organoids. We found that only kynurenine and no other tryptophan metabolite promotes the nuclear translocation of the transcription factor aryl hydrocarbon receptor (AHR). Blocking the interaction between AHR and kynurenine with CH223191 reduced the proliferation of colon cancer cells. Therefore, we propose that limiting cellular kynurenine or its downstream targets could present a new strategy to reduce the proliferation of MYC-dependent cancer cells.

Original languageEnglish (US)
Pages (from-to)1236-1251
Number of pages16
JournalGenes & development
Volume33
Issue number17-18
DOIs
StatePublished - Sep 1 2019

Fingerprint

Kynurenine
Tryptophan
Colonic Neoplasms
Arylformamidase
Large Neutral Amino Acid-Transporter 1
Aryl Hydrocarbon Receptors
Neoplasms
Organoids
Proto-Oncogenes
Enzymes
Tandem Mass Spectrometry
Glutamine
Transcription Factors
Epithelial Cells
High Pressure Liquid Chromatography
Amino Acids
Food

Keywords

  • AFMID
  • AHR
  • cancer
  • kynurenine
  • MYC
  • organoid
  • SLC1A5
  • SLC7A5
  • tryptophan metabolism

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Venkateswaran, N., Lafita-Navarro, M. C., Hao, Y. H., Kilgore, J. A., Perez-Castro, L., Braverman, J., ... Conacci-Sorrell, M. (2019). MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer. Genes & development, 33(17-18), 1236-1251. https://doi.org/10.1101/gad.327056.119

MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer. / Venkateswaran, Niranjan; Lafita-Navarro, M. Carmen; Hao, Yi Heng; Kilgore, Jessica A.; Perez-Castro, Lizbeth; Braverman, Jonathan; Borenstein-Auerbach, Nofit; Kim, Min; Lesner, Nicholas P.; Mishra, Prashant; Brabletz, Thomas; Shay, Jerry W.; DeBerardinis, Ralph J.; Williams, Noelle S.; Yilmaz, Omer H.; Conacci-Sorrell, Maralice.

In: Genes & development, Vol. 33, No. 17-18, 01.09.2019, p. 1236-1251.

Research output: Contribution to journalArticle

Venkateswaran, N, Lafita-Navarro, MC, Hao, YH, Kilgore, JA, Perez-Castro, L, Braverman, J, Borenstein-Auerbach, N, Kim, M, Lesner, NP, Mishra, P, Brabletz, T, Shay, JW, DeBerardinis, RJ, Williams, NS, Yilmaz, OH & Conacci-Sorrell, M 2019, 'MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer', Genes & development, vol. 33, no. 17-18, pp. 1236-1251. https://doi.org/10.1101/gad.327056.119
Venkateswaran N, Lafita-Navarro MC, Hao YH, Kilgore JA, Perez-Castro L, Braverman J et al. MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer. Genes & development. 2019 Sep 1;33(17-18):1236-1251. https://doi.org/10.1101/gad.327056.119
Venkateswaran, Niranjan ; Lafita-Navarro, M. Carmen ; Hao, Yi Heng ; Kilgore, Jessica A. ; Perez-Castro, Lizbeth ; Braverman, Jonathan ; Borenstein-Auerbach, Nofit ; Kim, Min ; Lesner, Nicholas P. ; Mishra, Prashant ; Brabletz, Thomas ; Shay, Jerry W. ; DeBerardinis, Ralph J. ; Williams, Noelle S. ; Yilmaz, Omer H. ; Conacci-Sorrell, Maralice. / MYC promotes tryptophan uptake and metabolism by the kynurenine pathway in colon cancer. In: Genes & development. 2019 ; Vol. 33, No. 17-18. pp. 1236-1251.
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AU - Braverman, Jonathan

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