Myoblast senescence in muscular dystrophy

Woodring E. Wright

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The limited proliferative capacity of normal diploid cells predicts that the utilization of cell divisions in vivo should reduce the lifespan of cells in culture. Because of the continuing demands for muscle regeneration in muscular dystrophy, myoblasts isolated from affected muscles should thus show a decrease in the number of cell divisions they are capable of expressing in culture. This hypothesis was tested by examining the proliferative capacity of myoblasts from different muscles for normal line 412 and dystrophic line 413 chickens of various ages. Prior to approx. 2 months of age, dystrophic myoblasts exhibited a relatively normal proliferative lifespan. By 5 months of age, myoblasts from the severely affected pectoralis major showed a 40% reduction in their proliferative potential, while myoblasts from the less affected posterior latissimus dorsi muscle showed a 25 % decrease in their cultured lifespan. The time course of the appearance of a decreased proliferative capacity only after the disease has been clinically manifested strongly supports it representing a secondary response rather than it being an intrinsic property of dystrophic myoblasts. A hypothesis for manipulating the pattern of stem cell division in order to increase the mass of muscle produced from a constant number of cell divisions is presented. If myoblast senescence and the consequent failure of muscle regeneration is a contributing factor in the progressive deterioration of muscle function in the disease, then this hypothesis might provide an important therapeutic strategy for ameliorating the course of muscular dystrophy.

Original languageEnglish (US)
Pages (from-to)343-354
Number of pages12
JournalExperimental Cell Research
Volume157
Issue number2
DOIs
StatePublished - 1985

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Muscular Dystrophies
Myoblasts
Muscles
Cell Division
Regeneration
Superficial Back Muscles
Diploidy
Chickens
Stem Cells
Cell Culture Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Myoblast senescence in muscular dystrophy. / Wright, Woodring E.

In: Experimental Cell Research, Vol. 157, No. 2, 1985, p. 343-354.

Research output: Contribution to journalArticle

Wright, Woodring E. / Myoblast senescence in muscular dystrophy. In: Experimental Cell Research. 1985 ; Vol. 157, No. 2. pp. 343-354.
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