Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck

Dylan T. Burnette, Lin Ji, Andrew W. Schaefer, Nelson A. Medeiros, Gaudenz Danuser, Paul Forscher

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The cell biological processes underlying axon growth and guidance are still not well understood. An outstanding question is how a new segment of the axon shaft is formed in the wake of neuronal growth cone advance. For this to occur, the highly dynamic, splayed-out microtubule (MT) arrays characteristic of the growth cone must be consolidated (bundled together) to form the core of the axon shaft. MT-associated proteins stabilize bundled MTs, but how individual MTs are brought together for initial bundling is unknown. Here, we show that laterally moving actin arcs, which are myosin II-driven contractile structures, interact with growing MTs and transport them from the sides of the growth cone into the central domain. Upon Myosin II inhibition, the movement of actin filaments and MTs immediately stopped and MTs unbundled. Thus, Myosin II-dependent compressive force is necessary for normal MT bundling in the growth cone neck.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalDevelopmental Cell
Volume15
Issue number1
DOIs
StatePublished - Jul 8 2008

Fingerprint

Myosin Type II
Growth Cones
Microtubules
Cones
Axons
Actins
Biological Phenomena
Microtubule-Associated Proteins
Actin Cytoskeleton
Growth

Keywords

  • CELLBIO
  • MOLNEURO

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck. / Burnette, Dylan T.; Ji, Lin; Schaefer, Andrew W.; Medeiros, Nelson A.; Danuser, Gaudenz; Forscher, Paul.

In: Developmental Cell, Vol. 15, No. 1, 08.07.2008, p. 163-169.

Research output: Contribution to journalArticle

Burnette, Dylan T. ; Ji, Lin ; Schaefer, Andrew W. ; Medeiros, Nelson A. ; Danuser, Gaudenz ; Forscher, Paul. / Myosin II Activity Facilitates Microtubule Bundling in the Neuronal Growth Cone Neck. In: Developmental Cell. 2008 ; Vol. 15, No. 1. pp. 163-169.
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