Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells

Hua Jia Diao, Wei Ching Low, Ulla Milbreta, Q. Richard Lu, Sing Yian Chew

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Remyelination in the central nervous system (CNS) is critical in the treatment of many neural pathological conditions. Unfortunately, the ability to direct and enhance oligodendrocyte (OL) differentiation and maturation remains limited. It is known that microenvironmental signals, such as substrate topography and biochemical signaling, regulate cell fate commitment. Therefore, in this study, we developed a nanofiber-mediated microRNA (miR) delivery method to control oligodendroglial precursor cell (OPC) differentiation through a combination of fiber topography and gene silencing. Using poly(ε-caprolactone) nanofibers, efficient knockdown of OL differentiation inhibitory regulators were achieved by either nanofiber alone (20-40%, p < 0.05) or the synergistic integration with miR-219 and miR-338 (up to 60%, p < 0.05). As compared to two-dimensional culture, nanofiber topography enhanced OPC differentiation by inducing 2-fold increase in RIP+ cells (p < 0.01) while the presence of miRs further enhanced the result to 3-fold (p < 0.001). In addition, nanofiber-mediated delivery of miR-219 and miR-338 promoted OL maturation by increasing the number of MBP+ cells significantly (p < 0.01). Taken together, the results demonstrate the efficacy of nanofibers in providing topographical cues and microRNA reverse transfection to direct OPC differentiation. Such scaffolds may find useful applications in directing oligodendrocyte differentiation and myelination for treatment of CNS pathological conditions that require remyelination.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalJournal of Controlled Release
Volume208
DOIs
StatePublished - Jun 28 2015

Keywords

  • Gene silencing
  • MicroRNA
  • Nanofiber
  • Oligodendrocyte precursor cell
  • Remyelination
  • Reverse transfection
  • Sustained release
  • Topography

ASJC Scopus subject areas

  • Pharmaceutical Science

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