Nanoparticulate cellular patches for cell-mediated tumoritropic delivery

Hao Cheng, Christian J. Kastrup, Renuka Ramanathan, Daniel J. Siegwart, Minglin Ma, Said R. Bogatyrev, Qiaobing Xu, Kathryn A. Whitehead, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.

Original languageEnglish (US)
Pages (from-to)625-631
Number of pages7
JournalACS Nano
Volume4
Issue number2
DOIs
StatePublished - Feb 23 2010

Fingerprint

Tumors
delivery
tumors
stem cells
Endothelial cells
Stem cells
cells
Nanoparticles
membranes
Medical nanotechnology
Tissue regeneration
nanoparticles
bone marrow
Cell membranes
regeneration
veins
Nanostructures
Bone
cancer
Membranes

Keywords

  • Cell therapy
  • Drug delivery
  • Endocytosis
  • Lipid raft
  • Membrane curvature
  • Tissue Engineering

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Cheng, H., Kastrup, C. J., Ramanathan, R., Siegwart, D. J., Ma, M., Bogatyrev, S. R., ... Anderson, D. G. (2010). Nanoparticulate cellular patches for cell-mediated tumoritropic delivery. ACS Nano, 4(2), 625-631. https://doi.org/10.1021/nn901319y

Nanoparticulate cellular patches for cell-mediated tumoritropic delivery. / Cheng, Hao; Kastrup, Christian J.; Ramanathan, Renuka; Siegwart, Daniel J.; Ma, Minglin; Bogatyrev, Said R.; Xu, Qiaobing; Whitehead, Kathryn A.; Langer, Robert; Anderson, Daniel G.

In: ACS Nano, Vol. 4, No. 2, 23.02.2010, p. 625-631.

Research output: Contribution to journalArticle

Cheng, H, Kastrup, CJ, Ramanathan, R, Siegwart, DJ, Ma, M, Bogatyrev, SR, Xu, Q, Whitehead, KA, Langer, R & Anderson, DG 2010, 'Nanoparticulate cellular patches for cell-mediated tumoritropic delivery', ACS Nano, vol. 4, no. 2, pp. 625-631. https://doi.org/10.1021/nn901319y
Cheng H, Kastrup CJ, Ramanathan R, Siegwart DJ, Ma M, Bogatyrev SR et al. Nanoparticulate cellular patches for cell-mediated tumoritropic delivery. ACS Nano. 2010 Feb 23;4(2):625-631. https://doi.org/10.1021/nn901319y
Cheng, Hao ; Kastrup, Christian J. ; Ramanathan, Renuka ; Siegwart, Daniel J. ; Ma, Minglin ; Bogatyrev, Said R. ; Xu, Qiaobing ; Whitehead, Kathryn A. ; Langer, Robert ; Anderson, Daniel G. / Nanoparticulate cellular patches for cell-mediated tumoritropic delivery. In: ACS Nano. 2010 ; Vol. 4, No. 2. pp. 625-631.
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